Efficient delivery of transgenes to human hair follicle progenitor cells using topical lipoplex

The topical delivery of transgenes to hair follicles has potential for treating disorders of the skin and hair. Here we show that the topical administration of liposome-DNA mixtures (lipoplex) to mouse skin and to human skin xenografts resulted in efficient in vivo transfection of hair follicle cell...

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Bibliographic Details
Published in:Nature biotechnology 2000-04, Vol.18 (4), p.420-423
Main Authors: Cotsarelis, George, Domashenko, Alevtina, Gupta, Sonya
Format: Article
Language:English
Subjects:
Acids
Agriculture
Animals
beta-Galactosidase - analysis
beta-Galactosidase - genetics
Bioinformatics
Biological and medical sciences
Biomedical and Life Sciences
Biomedical Engineering/Biotechnology
Biomedicine
Biotechnology
Cation Exchange Resins
Cell Transplantation - methods
Deoxyribonucleic acid
DNA
Drug Carriers
Efficiency
Follicles
Fundamental and applied biological sciences. Psychology
Gene therapy
Gene Transfer Techniques
Hair
Hair Follicle - cytology
Hair Follicle - transplantation
Health. Pharmaceutical industry
Humans
Industrial applications and implications. Economical aspects
Life Sciences
Lipids
lipoplex
Liposomes
Male
Mice
Mice, Inbred ICR
Mice, SCID
Scalp
Skin
Skin Transplantation
Stem cells
Stem Cells - cytology
Stem Cells - physiology
Transfection - methods
Transplantation, Heterologous
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Summary:The topical delivery of transgenes to hair follicles has potential for treating disorders of the skin and hair. Here we show that the topical administration of liposome-DNA mixtures (lipoplex) to mouse skin and to human skin xenografts resulted in efficient in vivo transfection of hair follicle cells. Transfection depended on liposome composition, and occurred only at the onset of a new growing stage of the hair cycle. Manipulating the hair follicle cycle with depilation and retinoic acid treatment resulted in nearly 50% transfection efficiency–defined as the proportion of transfected, newly growing follicles within the xenograft. Transgenes administered in this fashion are selectively expressed in hair progenitor cells and therefore have the potential to affect the characteristics of the follicle. These findings form a foundation for the future use of topical lipoplex applications to alter hair follicle phenotype and treat diseases of the hair and skin.
ISSN:1087-0156
1546-1696
DOI:10.1038/74480