The effect of pretreatment with S,S,S-tributyl phosphorotrithioate on deltamethrin resistance and carboxylesterase activity in Aphis gossypii (Glover) (Homoptera: Aphididae)

• Published in: Pesticide biochemistry and physiology 2010-10, Vol.98 (2), p.296-299
• Main Authors: Chang, Jing, Cao, Chuan-Wang, Gao, Xi-Wu
• Format: Article
• Language: English
• Subjects: Aphididae; Aphis gossypii; Aphis gossypii (Glover); Biological and medical sciences; Carboxylesterase; Fundamental and applied biological sciences. Psychology; Homoptera; Insecticide resistance; Phytopathology. Animal pests. Plant and forest protection; Protozoa. Invertebrates; Pyrethroid; S,S,S-tributyl phosphorotrithioate; Synergism
• ISSN: 0048-3575; 1095-9939
• DOI: 10.1016/j.pestbp.2010.06.021

The synergism of S,S,S-tributyl phosphorotrithioate (DEF) and its effect on carboxylesterase activity were investigated in deltamethrin-selected resistant (DRR) and susceptible (DSS) strains of cotton aphids, Aphis gossypii (Glover). Compared to the DSS strain, the DRR strain showed 23,900-fold resistance to deltamethrin, and 7560- and 99-fold cross-resistance to bifenthrin and ethofenprox, respectively. The synergist, DEF, increased the toxicity of both deltamethrin and bifenthrin, but not of ethofenprox when DEF was pretreated of 15 h. DEF exhibited significant inhibition on the carboxylesterase activity in the DRR strain, but no significant effect on that of the DSS strain in vitro. After the cotton aphids exposing to DEF, the carboxylesterase activity decreased gradually until 15 h and then gradually recovered until 24 h in the DRR strain, which fluctuated according to the effect of DEF on the deltamethrin toxicity detected using DEF pretreatment in the DRR strain. Therefore, our studies suggested that the effect of DEF on carboxylesterase was associated with deltamethrin resistance in the DRR strain.