Transgenic mice expressing bacterial phytase as a model for phosphorus pollution control

We have developed transgenic mouse models to determine whether endogenous expression of phytase transgenes in the digestive tract of monogastric animals can increase the bioavailability of dietary phytate, a major but indigestible form of dietary phosphorus. We constructed phytase transgenes compose...

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Bibliographic Details
Published in:Nature biotechnology 2001-05, Vol.19 (5), p.429-433
Main Authors: Phillips, John P, Forsberg, Cecil W, Golovan, Serguei P, Hayes, M. Anthony
Format: Article
Language:English
Subjects:
6-Phytase - genetics
Acid Phosphatase - genetics
Agriculture
Animals
appA gene
Bioavailability
Bioinformatics
Biological and medical sciences
Biomedical and Life Sciences
Biomedical Engineering/Biotechnology
Biomedicine
Biotechnology
Blotting, Northern
E coli
Electrophoresis, Polyacrylamide Gel
Environmental impact
Environmental Pollution - prevention & control
Escherichia coli
Escherichia coli Proteins
Exocrine glands
Feces - chemistry
Feeds
Female
Females
Fundamental and applied biological sciences. Psychology
Genetic engineering
Genetic technics
Immunohistochemistry
Life Sciences
Male
Manure - analysis
Manures
Methods. Procedures. Technologies
Mice
Mice, Transgenic - physiology
Parotid Gland - enzymology
Phosphates
Phosphorus
Phosphorus - metabolism
Pollution
Pollution control
Poultry
Proteins
Publishing
Rats
Submandibular Gland - enzymology
Transgenic animals
Transgenic animals and transgenic plants
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Summary:We have developed transgenic mouse models to determine whether endogenous expression of phytase transgenes in the digestive tract of monogastric animals can increase the bioavailability of dietary phytate, a major but indigestible form of dietary phosphorus. We constructed phytase transgenes composed of the appA phytase gene from Escherichia coli regulated for expression in salivary glands by the rat R15 proline-rich protein promoter or by the mouse parotid secretory protein promoter. Transgenic phytase is highly expressed in the parotid salivary glands and secreted in saliva as an enzymatically active 55 kDa glycosylated protein. Expression of salivary phytase reduces fecal phosphorus by 11%. These results suggest that the introduction of salivary phytase transgenes into monogastric farm animals offers a promising biological approach to relieving the requirement for dietary phosphate supplements and to reducing phosphorus pollution from animal agriculture.
ISSN:1087-0156
1546-1696
DOI:10.1038/88091