A physical map of the human Y chromosome

The non-recombining region of the human Y chromosome (NRY), which comprises 95% of the chromosome, does not undergo sexual recombination and is present only in males. An understanding of its biological functions has begun to emerge from DNA studies of individuals with partial Y chromosomes, coupled...

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Bibliographic Details
Published in:Nature (London) 2001-02, Vol.409 (6822), p.943-945
Main Authors: Page, David C, Tilford, Charles A, Kuroda-Kawaguchi, Tomoko, Skaletsky, Helen, Rozen, Steve, Brown, Laura G, Rosenberg, Michael, McPherson, John D, Wylie, Kristine, Sekhon, Mandeep, Kucaba, Tamara A, Waterston, Robert H
Format: Article
Language:English
Subjects:
Chromosomes
Chromosomes, Artificial, Bacterial
Cloning
Deoxyribonucleic acid
DNA
Euchromatin
Gene Amplification
Genes
Genome, Human
Genomics
Heterochromatin
Humanities and Social Sciences
Humans
letters-to-nature
Male
multidisciplinary
Physical Chromosome Mapping - methods
Radiation Hybrid Mapping
Science
Sequence Tagged Sites
Y Chromosome
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Summary:The non-recombining region of the human Y chromosome (NRY), which comprises 95% of the chromosome, does not undergo sexual recombination and is present only in males. An understanding of its biological functions has begun to emerge from DNA studies of individuals with partial Y chromosomes, coupled with molecular characterization of genes implicated in gonadal sex reversal, Turner syndrome, graft rejection and spermatogenic failure. But mapping strategies applied successfully elsewhere in the genome have faltered in the NRY, where there is no meiotic recombination map and intrachromosomal repetitive sequences are abundant. Here we report a high-resolution physical map of the euchromatic, centromeric and heterochromatic regions of the NRY and its construction by unusual methods, including genomic clone subtraction and dissection of sequence family variants. Of the map's 758 DNA markers, 136 have multiple locations in the NRY, reflecting its unusually repetitive sequence composition. The markers anchor 1,038 bacterial artificial chromosome clones, 199 of which form a tiling path for sequencing.
ISSN:0028-0836
1476-4687
DOI:10.1038/35057170