An ouabain-like factor is secreted from immortalized hypothalamic cells in an aldosterone-dependent manner

► Brain ouabain is a key in blood pressure regulation upon excess sodium intake. ► Ouabain is produced by immortalized N1 cells, which are of hypothalamic origin. ► Aldosterone augments ouabain release in cultured N1 cells. ► Ouabain release is blocked by eplerenone, a mineralocorticoid receptor blo...

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Bibliographic Details
Published in:Neurochemistry international 2011-08, Vol.59 (2), p.104-108
Main Authors: Yoshika, Masamichi, Komiyama, Yutaka, Takahashi, Hakuo
Format: Article
Language:English
Subjects:
Aldosterone
Aldosterone - physiology
Animals
Biological and medical sciences
Cardenolides
Cell Line, Transformed
Chromatography, High Pressure Liquid
ELISA
Endogenous digitalis
Enzyme-Linked Immunosorbent Assay
Eplerenone
Fundamental and applied biological sciences. Psychology
Hypothalamus - cytology
Hypothalamus - secretion
Immortalized hypothalamic cell
Mice
Ouabain
Saponins - secretion
Vertebrates: nervous system and sense organs
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Summary:► Brain ouabain is a key in blood pressure regulation upon excess sodium intake. ► Ouabain is produced by immortalized N1 cells, which are of hypothalamic origin. ► Aldosterone augments ouabain release in cultured N1 cells. ► Ouabain release is blocked by eplerenone, a mineralocorticoid receptor blocker. ► Thus, there is aldosterone-regulated secretion of ouabain by the hypothalamus. Ouabain-like factor (OLF) modulates blood pressure via sodium pump inhibition in the central nervous system and in the peripheral circulation. Ouabain-like factor (OLF) is thought to be produced in the adrenal gland and hypothalamus, and it may relate locally to the renin–angiotensin–aldosterone system. However, the evidence for the latter was obtained from in vivo experiments using animals. In the present study, we investigated ouabain production in the immortalized hypothalamic cell line N1. First, cell culture supernatant was collected from the immortalized hypothalamic cell line N1 at 0.5, 4, 8, and 24h. A newly developed enzyme-linked immunosorbent assay (ELISA) that used anti-ouabain antibody showed that immunoreactivity in the supernatant was increased significantly at 24 vs. 0.5h (0.01±0.004 vs. 0.16±0.033pmol/mg protein, p
ISSN:0197-0186
1872-9754
DOI:10.1016/j.neuint.2011.04.019