Investigation of the Photodynamic Effects of Curcumin Against Candida albicans

This study describes the association of curcumin with light emitting diode (LED) for the inactivation of Candida albicans. Suspensions of Candida were treated with nine curcumin concentrations and exposed to LED at different fluences. The protocol that showed the best outcomes for Candida inactivati...

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Bibliographic Details
Published in:Photochemistry and photobiology 2011-07, Vol.87 (4), p.895-903
Main Authors: Dovigo, Lívia N., Pavarina, Ana Cláudia, Ribeiro, Ana Paula D., Brunetti, Iguatemy L., Costa, Carlos Alberto de S., Jacomassi, Denis P., Bagnato, Vanderlei S., Kurachi, Cristina
Format: Article
Language:English
Subjects:
Animals
Antifungal Agents - chemistry
Antifungal Agents - pharmacology
Biofilms - drug effects
Biofilms - growth & development
Biofilms - radiation effects
Cancer
Candida albicans
Candida albicans - drug effects
Candida albicans - growth & development
Candida albicans - radiation effects
Candidiasis - drug therapy
Candidiasis - microbiology
Cell Line
Curcumin - chemistry
Curcumin - pharmacology
Dose-Response Relationship, Drug
Fluorescence
Humans
Infections
Light
Mice
Nosocomial infections
Photobleaching - radiation effects
Photochemotherapy - methods
Photosensitizing Agents - chemistry
Photosensitizing Agents - pharmacology
Plankton - drug effects
Plankton - growth & development
Plankton - radiation effects
Spectrometry, Fluorescence
Studies
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Summary:This study describes the association of curcumin with light emitting diode (LED) for the inactivation of Candida albicans. Suspensions of Candida were treated with nine curcumin concentrations and exposed to LED at different fluences. The protocol that showed the best outcomes for Candida inactivation was selected to evaluate the effect of the preirradiation time (PIT) on photodynamic therapy (PDT) effectiveness, the uptake of curcumin by C. albicans cells and the possible involvement of singlet oxygen in the photodynamic action. Curcumin‐mediated PDT was also assessed against biofilms. In addition to the microbiological experiments, similar protocols were tested on a macrophage cell line and the effect was evaluated by Methyltetrazolium assay (MTT) and SEM analysis. The optical properties of curcumin were investigated as a function of illumination fluence. When compared with the control group, a statistically significant reduction in C. albicans viability was observed after PDT (P 
ISSN:0031-8655
1751-1097
DOI:10.1111/j.1751-1097.2011.00937.x