Ratiometric Detection of Mitochondrial Thiols with a Two-Photon Fluorescent Probe

We report a ratiometric two-photon probe (SSH-Mito) for mitochondrial thiols. This probe shows a marked blue-to-yellow emission color change in response to RSH, a significant two-photon cross section, good mitochondrial thiol selectivity, low cytotoxicity, and insensitivity to pH over the biological...

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Bibliographic Details
Published in:Journal of the American Chemical Society 2011-07, Vol.133 (29), p.11132-11135
Main Authors: Lim, Chang Su, Masanta, Goutam, Kim, Hyung Joong, Han, Ji Hee, Kim, Hwan Myung, Cho, Bong Rae
Format: Article
Language:English
Subjects:
Fluorescent Dyes - chemistry
HeLa Cells
Humans
Microscopy, Fluorescence, Multiphoton
Mitochondria - chemistry
Photons
Sulfhydryl Compounds - analysis
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Summary:We report a ratiometric two-photon probe (SSH-Mito) for mitochondrial thiols. This probe shows a marked blue-to-yellow emission color change in response to RSH, a significant two-photon cross section, good mitochondrial thiol selectivity, low cytotoxicity, and insensitivity to pH over the biologically relevant pH range, allowing the direct visualization of RSH levels in live cells as well as in living tissues at 90–190 μm depth without interference from other biologically relevant species through the use of two-photon microscopy.
ISSN:0002-7863
1520-5126
DOI:10.1021/ja205081s