High-resolution extracellular stimulation of dispersed hippocampal culture with high-density CMOS multielectrode array based on non-Faradaic electrodes

We introduce a method to electrically stimulate individual neurons at single-cell resolution in arbitrary spatiotemporal patterns with precise control over stimulation thresholds. By exploiting a custom microelectronic chip, up to 65,000 non-Faradaic electrodes can be uniquely addressed with electro...

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Bibliographic Details
Published in:Journal of neural engineering 2011-08, Vol.8 (4), p.044003-1-6
Main Authors: Lei, N, Ramakrishnan, S, Shi, P, Orcutt, J S, Yuste, R, Kam, L C, Shepard, K L
Format: Article
Language:English
Subjects:
Algorithms
Animals
Arrays
Calcium Signaling - physiology
Calibration
Cells, Cultured
Chips
CMOS
Culture
Density
Dispersion
Electric Stimulation - methods
Electrodes
Equipment Design
Extracellular Space - physiology
Hippocampus - cytology
Hippocampus - physiology
Image Processing, Computer-Assisted
Immunohistochemistry
Microelectrodes
Microscopy, Fluorescence
Neurons - physiology
Rats
Rats, Sprague-Dawley
Semiconductors
Stimulation
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Summary:We introduce a method to electrically stimulate individual neurons at single-cell resolution in arbitrary spatiotemporal patterns with precise control over stimulation thresholds. By exploiting a custom microelectronic chip, up to 65,000 non-Faradaic electrodes can be uniquely addressed with electrode density exceeding 6500 electrodes mm(-2). We demonstrate extracellular stimulation of dispersed primary hippocampal neuronal cultures using the chip at single-cell resolution.
ISSN:1741-2560
1741-2552
DOI:10.1088/1741-2560/8/4/044003