In vitro expression profiling of myostatin, follistatin, decorin and muscle-specific transcription factors in adult caprine contractile myotubes

Skeletal muscle is one of the several adult postmitotic tissues that retain the capacity to regenerate, which relies on a population of quiescent precursors, termed satellite cells. Proliferation and differentiation of myoblasts to form mature myotubes in vitro has been a valuable tool in the charac...

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Bibliographic Details
Published in:Journal of muscle research and cell motility 2011-08, Vol.32 (1), p.23-30
Main Authors: Tripathi, A. K., Ramani, U. V., Rank, D. N., Joshi, C. G.
Format: Article
Language:English
Subjects:
Animal Anatomy
Animals
Biomedical and Life Sciences
Biomedicine
Cell Biology
Cell Differentiation - genetics
Cells, Cultured
Decorin - genetics
Decorin - metabolism
Follistatin - genetics
Follistatin - metabolism
Gene Expression Profiling
Gene Expression Regulation
Goats
Histology
Humans
Life Sciences
Morphology
Muscle Development - genetics
Muscle Development - physiology
Muscle Fibers, Skeletal - cytology
Muscle Fibers, Skeletal - metabolism
MyoD Protein - genetics
MyoD Protein - metabolism
Myogenic Regulatory Factor 5 - genetics
Myogenic Regulatory Factor 5 - metabolism
Myogenin - genetics
Myogenin - metabolism
Myostatin - genetics
Myostatin - metabolism
Original Paper
Proteomics
Satellite Cells, Skeletal Muscle - cytology
Satellite Cells, Skeletal Muscle - metabolism
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Summary:Skeletal muscle is one of the several adult postmitotic tissues that retain the capacity to regenerate, which relies on a population of quiescent precursors, termed satellite cells. Proliferation and differentiation of myoblasts to form mature myotubes in vitro has been a valuable tool in the characterization of the cellular events during myogenesis, which is a multistep process starting with progenitor cell proliferation, followed by their exit from the cell cycle, differentiation, alignment, and fusion to form multinucleated myotubes. A typical feature during muscle differentiation is the variation in expression of various genes along with myogenic factors. In this experiment, mRNA level of myostatin, follistatin, decorin and three muscle-specific transcription factors in adult caprine contractile myotubes have been studied through quantitative real time PCR. We observed that the expression level of myostatin, decorin, Myf5 and myogenin transcripts were significantly higher in contractile myotubes compared to myoblast monolayer ( P  
ISSN:0142-4319
1573-2657
DOI:10.1007/s10974-011-9245-x