Physical and functional cooperation of neural cell adhesion molecule and β1-integrin in neurite outgrowth induction

Abstract Neural cell adhesion molecule (NCAM) and β1-integrin are both involved in cell differentiation, with changes in the expression of these two molecules correlating with changes in the malignancy of tumor cells. There is a known functional correlation between NCAM and β1-integrin in adhesion a...

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Bibliographic Details
Published in:Journal of clinical neuroscience 2011-08, Vol.18 (8), p.1109-1113
Main Authors: Petridis, Athanasios K, Nikolopoulos, Sotiris N, El-Maarouf, Abderrahman
Format: Article
Language:English
Subjects:
Analysis of Variance
Animals
Animals, Newborn
Antibodies - pharmacology
Cancer
Cell differentiation
Cell Line
Cerebral Ventricles - cytology
Cricetinae
Cricetulus
Enzyme Inhibitors - pharmacology
Fibronectins - pharmacology
Glycoside Hydrolases - pharmacology
Humans
Immunoprecipitation
In Vitro Techniques
Integrin beta1 - genetics
Integrin beta1 - immunology
Integrin beta1 - metabolism
Integrins
Mice
Neural cell adhesion molecule
Neural Cell Adhesion Molecules - genetics
Neural Cell Adhesion Molecules - immunology
Neural Cell Adhesion Molecules - metabolism
Neurites - drug effects
Neurites - metabolism
Neuroblastoma - pathology
Neurofilament Proteins - metabolism
Neurology
Neurons - cytology
Neurons - drug effects
Phospholipase C gamma - metabolism
Transfection
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Summary:Abstract Neural cell adhesion molecule (NCAM) and β1-integrin are both involved in cell differentiation, with changes in the expression of these two molecules correlating with changes in the malignancy of tumor cells. There is a known functional correlation between NCAM and β1-integrin in adhesion and also neurite outgrowth in tumor cells. In the present study, we used immunostaining and immunoprecipitation studies to demonstrate that isoform 120 of NCAM associates physically as well as functionally with β1-integrin in the induction of neurite outgrowth in SH-SY5Y-human neuroblastoma cells. The interaction between these two molecules is mandatory for neurite outgrowth. NCAM blockage completely inhibits the effects of β1-integrin on neurite outgrowth. These findings further our understanding of the interactions between NCAMs and integrins in malignancy.
ISSN:0967-5868
1532-2653
DOI:10.1016/j.jocn.2010.12.047