Enzyme Activation and Inactivation Induced by Low Doses of Irradiation

Activation phenomenon has been observed with two sets of enzymes under the conditions of low dosage irradiation. Activation was registered for angiotensin-converting enzyme under in vitro γ-irradiation (0.662 MeV, pulse duration approx 10s) at dose levels of 1-3 Gy and under X-ray irradiation (appro...

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Bibliographic Details
Published in:Applied biochemistry and biotechnology 2000-07, Vol.88 (1-3), p.243-255
Main Authors: Orlova, Marina A, Kost, Olga A, Gribkov, Vladimir A, Gazaryan, Irina G, Dubrovsky, Aleksandr V, Egorov, Valeriy A, Troshina, Nina N
Format: Article
Language:English
Subjects:
Biochemistry
Enzymes
Inactivation
Irradiation
Kinetics
Nicotiana tabacum
Radiation
Studies
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Summary:Activation phenomenon has been observed with two sets of enzymes under the conditions of low dosage irradiation. Activation was registered for angiotensin-converting enzyme under in vitro γ-irradiation (0.662 MeV, pulse duration approx 10s) at dose levels of 1-3 Gy and under X-ray irradiation (approx 9 keV, pulse duration approx 10^sup -9^s) at dose levels of 2×10^sup -5^ Gy. An activation effect has also occurred for native and recombinant horseradish peroxidase and tobacco peroxidase under γ-irradiation. The phenomenon observed is rationalized in terms of a kinetic model suggesting the existence of at least one activated enzyme conformation induced by radiolysis. The activity oscillations registered in dense plasma focus experiments were rationalized using the same model with the corresponding kinetic equation converted into the form describing the decaying oscillations caused by exciting force. The model analysis is presented.[PUBLICATION ABSTRACT]
ISSN:0273-2289
0273-2289
1559-0291
DOI:10.1385/ABAB:88:1-3:243