What makes Grande1 retrotransposon different?

Grande1 elements constitute a family of Ty3 retrotransposons present in the Zea genus in more than 1000 copies in Zea diploperennis and maize. The sequences of three Grande1 flanking regions, two from Z. diploperennis and one from maize, reveal transposable elements as insertion targets, suggesting...

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Bibliographic Details
Published in:Genetica 1997-01, Vol.100 (1-3), p.15-28
Main Authors: Martinez-Izquierdo, J.A. (Centre d'Investigacio i Desenvolupament CSIC, Barcelona (Spain). Dept. de Genetica Molecular), Garcia-Martinez, J, Vicient, C.M
Format: Article
Language:English
Subjects:
ADN
Antisense
Base Sequence
Corn
DNA
DNA Methylation
GENE
GENE TRANSPOSITION
GENES
Genome, Plant
Molecular Sequence Data
NUCLEOTIDE SEQUENCE
Repetitive Sequences, Nucleic Acid
Retroelements
SECUENCIA NUCLEOTIDICA
Sequence Homology, Nucleic Acid
SEQUENCE NUCLEOTIDIQUE
Transcription, Genetic
TRANSPOSICION DE GENES
TRANSPOSITION DE GENES
Zea
ZEA DIPLOPERENNIS
ZEA MAYS
Zea mays - genetics
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Summary:Grande1 elements constitute a family of Ty3 retrotransposons present in the Zea genus in more than 1000 copies in Zea diploperennis and maize. The sequences of three Grande1 flanking regions, two from Z. diploperennis and one from maize, reveal transposable elements as insertion targets, suggesting a preferential integration of Grande1 elements into other transposable elements. These retrotransposons are remarkable for their large size of around 14 kb, which is a consequence of a very large 3' region of more than 7 kb. Atypical entities within this region are two arrays of unrelated tandem repeats with potential stable stem-loop structures. A large portion of the same region is occupied by ORFs, although only ORF23, whose function is unknown, is presumably transcribed in antisense orientation to the reverse transcriptase ORF. Only ORF23 has a codon usage similar to the one tabulated for highly-expressed maize genes. Correspondingly, the transcript of 900 b that hybridizes with ORF23 probes is found in all the maize tissues explored. This is despite the high level of methylation in the DNA of Grande1. Genomic RNA has not been detected in any tissue or situation studied, probably reflecting a non-functional retrotransposon. The origin of ORF23 and the remainder 3' region might be due to a transduction event.
ISSN:0016-6707
1573-6857
DOI:10.1023/A:1018332218319