Transient receptor potential (TRP) A1 activated currents in TRPV1 and cholecystokinin-sensitive cranial visceral afferent neurons

• Published in: Brain research 2011-04, Vol.1383, p.36-42
• Main Authors: Choi, Myung-Jin, Jin, Zhenhua, Park, Yong Seek, Rhee, Young Kyoung, Jin, Young-Ho
• Format: Article
• Language: English
• Subjects: agonists; allyl isothiocyanate; Animals; Ankyrins - metabolism; antagonists; Biological and medical sciences; brain; Calcium Channels - metabolism; capsaicin; Cholecystokinin; Cholecystokinin - metabolism; food intake; Fundamental and applied biological sciences. Psychology; ingredients; Neurology; Neurons, Afferent - metabolism; Nodose Ganglion - metabolism; pain; Patch-Clamp Techniques; Peripheral nervous system. Autonomic nervous system. Neuromuscular transmission. Ganglionic transmission. Electric organ; Rats; Rats, Sprague-Dawley; Satiety; Satiety Response - physiology; sensory neurons; Spices; TRPA1 Cation Channel; TRPC Cation Channels; TRPV Cation Channels - metabolism; Vagus nerve; Vertebrates: nervous system and sense organs; Visceral afferent neuron
• ISSN: 0006-8993; 1872-6240
• DOI: 10.1016/j.brainres.2011.02.009

Abstract Culinary use of the pungent spices has potential health benefits including a reduction in food intake. Pungent spices often contain ingredients that activate members of the transient receptor potential (TRP) family A1 and evoke pain from capsaicin-sensitive somatosensory neurons. TRPA1 channel have also been identified on cranial visceral afferent neurons but their distribution and functional contributions are poorly understood. Visceral vagal neurons transduce mechanical and chemical signals from peripheral organs to the nucleus tractus solitarii. Many capsaicin-sensitive vagal afferents participate in peripheral satiety signaling that includes cholecystokinin (CCK) sensitive neurons. To assess signaling, the TRPA1 selective agonist allyl isothiocyanate (AITC) was tested together with CCK and capsaicin (200 nM), a TRPV1 specific agonist. In isolated nodose neurons, AITC (0.05–0.2 mM) evoked concentration-dependent inward currents in 38% of the tested neurons. The TRPA1 specific antagonist HC-030031 (10 μM) blocked AITC responses. TRPA1 responses were mixed across neurons that were capsaicin-sensitive and -insensitive. However CCK evoked inward currents only on capsaicin-sensitive neurons and 28% of the CCK-sensitive neurons expressed TRPA1. Our results indicate that TRPA1 is co-expressed with TRPV1 in CCK-sensitive nodose neurons. The findings indicate a potential mechanism by which spices can act within cranial visceral afferent pathways mediating satiety and contribute to the reduction of the food intake associated with spiced diets.