Isolation and characterization of Doublesex homologues in the Bactrocera species: B. dorsalis (Hendel) and B. correcta (Bezzi) and their putative promoter regulatory regions

Doublesex (dsx) is a double-switch gene at the bottom of the somatic sex-determination hierarchy which regulates sexual dimorphism in many insects. Here, Drosophila melanogaster homologues of dsx were isolated in two Bactrocera species, the oriental fruit fly, B. dorsalis, and the guava fruit fly, B...

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Bibliographic Details
Published in:Genetica 2011, Vol.139 (1), p.113-127
Main Authors: Permpoon, Rattiya, Aketarawong, Nidchaya, Thanaphum, Sujinda
Format: Article
Language:English
Subjects:
Alternative Splicing
Amino Acid Sequence
Animal Genetics and Genomics
Animals
Bactrocera
Bactrocera correcta
Bactrocera dorsalis
Base Sequence
Biomedical and Life Sciences
Conserved Sequence
DNA-Binding Proteins - genetics
Drosophila melanogaster - genetics
Drosophila Proteins - genetics
dsx core promoter
Evolution, Molecular
Evolutionary Biology
Female
Fruits
Gene expression
Genetic sexing
Human Genetics
Insect Proteins - genetics
Insects
Life Sciences
Male
Microbial Genetics and Genomics
Molecular Sequence Data
Pest Control, Biological
Phylogeny
Plant Genetics and Genomics
Promoter Regions, Genetic
Research projects
Sex Determination Processes - genetics
SI-Molecular Technologies to Improve SIT
SIT
Tephritidae - genetics
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Summary:Doublesex (dsx) is a double-switch gene at the bottom of the somatic sex-determination hierarchy which regulates sexual dimorphism in many insects. Here, Drosophila melanogaster homologues of dsx were isolated in two Bactrocera species, the oriental fruit fly, B. dorsalis, and the guava fruit fly, B. correcta. Results of RT-PCR analysis suggests that both the B. dorsalis dsx (Bd1dsx) and B. correcta dsx (Bcdsx) genes are transcribed and sex-specifically spliced in accordance with the Drosophila sex-specific splicing mechanism. The cDNA sequences shared a high degree of similarity at the nucleotide level among the Bactrocera species. Structurally conserved domains for DNA-binding and oligomerization were observed in all transcripts suggesting that their proteins function as transcriptional factors for downstream sex-specific gene expression. A purine-rich element (PRE) and four repeat elements (dsxRE) for TRA/TRA-2 binding sites were also found in the 3′ untranslated regions (UTR) of both the female Bd1dsx and Bcdsx mRNAs. Notably, a putative core promoter was revealed in Bd1dsx, being probably the first dsx promoter discovered in the tephritid flies.
ISSN:0016-6707
1573-6857
DOI:10.1007/s10709-010-9508-2