Bioactivity guided isolation of mosquito larvicide from Piper longum

<正>Objective:To isolate the larvicidal component from the fruits of Piper longum（P.longum） against the filariasis vector,Culex quinquefasciatus（C.quinquefasciatus）.Methods:Pulverized fruits of P.longum were subjected to soxhlet extraction using series of organic solvents of increasing...

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Bibliographic Details
Published in:Asian Pacific journal of tropical medicine 2011-02, Vol.4 (2), p.112-116
Main Authors: SK, Madhu, VA, Vijayan, AK, Shaukath
Format: Article
Language:English
Subjects:
Animals
Bioassay
Biological Assay
Chemical Fractionation
Chromatography, Liquid
Culex
Culex - drug effects
Culex quinquefasciatus
Filariasis
Insecticides - chemistry
Insecticides - isolation & purification
Insecticides - pharmacology
Larva - drug effects
Larvicide
longum
Piper
Piper - chemistry
Piper longum
Pipyahyine
Plant Extracts - chemistry
Plant Extracts - isolation & purification
Plant Extracts - pharmacology
quinquefasciatus
Survival Analysis
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Summary:<正>Objective:To isolate the larvicidal component from the fruits of Piper longum（P.longum） against the filariasis vector,Culex quinquefasciatus（C.quinquefasciatus）.Methods:Pulverized fruits of P.longum were subjected to soxhlet extraction using series of organic solvents of increasing polarity.All the solvent extracts were verified for their larvicidal efficacy against 4th instar larvae of C.quinquefasciatus employing standard WHO procedure.Bioassayguided fractionation through column chromatography lead to the isolation of a bioactive amide, pipyahyine from the petroleum ether extract.Results:Petroleum ether extract was found to be the most active fraction among all the extracts tested with LC50 and LC90 being 1.03 and 2.04 ppm respectively.Whereas,pipyahyine,an isolated component of the same fraction was found to be even more effective than the parent extract in terms of LC50 being 0.58 and 1.88 ppm respectively. Conclusions:From the results,it is evident that P.longum can be considered as a powerful arsenal for the control of mosquito population.
ISSN:1995-7645
2352-4146
DOI:10.1016/S1995-7645(11)60048-5