Floating cells with stem cell properties in gastric cell line SGC-7901

AIMSTo obtain floating spheres from the adherent gastric cancer cell line SGC-7901 and to analyze the properties of the spheres. METHODSSerum-free medium was applied to cultured SGC-7901 cells. Limiting dilution assay, tumor formation assay, microarray analysis, and real-time fluorescent quantitativ...

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Bibliographic Details
Published in:Tumori 2011-05, Vol.97 (3), p.393-399
Main Authors: Zheng, QiuHong, Gong, FuSheng, Xu, YangMei, Zheng, TianRong, Ying, MinGang
Format: Article
Language:English
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Summary:AIMSTo obtain floating spheres from the adherent gastric cancer cell line SGC-7901 and to analyze the properties of the spheres. METHODSSerum-free medium was applied to cultured SGC-7901 cells. Limiting dilution assay, tumor formation assay, microarray analysis, and real-time fluorescent quantitative PCR were used to test the stem cell properties of the spheres. RESULTSA subpopulation of SGC-7901 cells formed floating spheres in serum-free medium. These cells showed enhanced tumorigenic ability and also highly expressed certain stem-cell-associated proteins. CONCLUSIONSWe successfully propagated floating spheres with stem cell properties in the SGC-7901 gastric cell line.
ISSN:0300-8916
DOI:10.1700/912.10040