Multiresidue Analysis of Multiclass Plant Growth Regulators in Grapes by Liquid Chromatography/Tandem Mass Spectrometry

A selective and rapid multiresidue analysis method is presented for simultaneous estimation of 12 plant growth regulators (PGRs), namely, auxins (indol-3-acetic acid, indol-3-butyric acid, and naphthyl acetic acid), cytokinins (kinetin, zeatin, and 6-benzyladenine), gibberellic acid (GA3), abscisic...

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Bibliographic Details
Published in:Journal of AOAC International 2011-05, Vol.94 (3), p.968-977
Main Authors: OULKAR, Dasharath P, BANERJEE, Kaushik, GHASTE, Manoj S, RAMTEKE, Sahadeo D, NAIK, Dattatraya G, PATIL, Shubhangi B, JADHAV, Manjusha R, ADSULE, Pandurang G
Format: Article
Language:English
Subjects:
Biological and medical sciences
Chemical properties
Chromatography, Liquid - methods
Food industries
Fruit - chemistry
Fruit and vegetable industries
Fundamental and applied biological sciences. Psychology
Genetic aspects
Grapes
Identification and classification
Liquid chromatography
Mass spectrometry
Methods
Molecular Structure
Physiological aspects
Plant Growth Regulators - chemistry
Plant Growth Regulators - classification
Plant hormones
Tandem Mass Spectrometry - methods
Vitis - chemistry
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Summary:A selective and rapid multiresidue analysis method is presented for simultaneous estimation of 12 plant growth regulators (PGRs), namely, auxins (indol-3-acetic acid, indol-3-butyric acid, and naphthyl acetic acid), cytokinins (kinetin, zeatin, and 6-benzyladenine), gibberellic acid (GA3), abscisic acid, and synthetic compounds, namely, forchlorfenuron, paclobutrazole, isoprothiolane, and 2,4-dichlorophenoxy acetic acid (2,4-D) in bud sprouts and grape berries at the development stages of 2-3 and 6-8 mm diameters, which are the critical phases when exogenous application of PGRs may be necessary to achieve desired grape quality and yield. The sample preparation method involved extraction of plant material with acidified methanol (50%) by homogenization for 2 min at 15000 rpm. The pH of the extract was enhanced up to 6 by adding ammonium acetate, followed by homogenization and centrifugation. The supernatant extract was cleaned by SPE on an Oasis HLB cartridge (200 mg, 6 cc). The final extract was measured directly by LC/MS/MS with electrospray ionization in positive mode, except for 2,4-D, GA3, and abscisic acid extracts, which required analysis in negative mode. Quantification by multiple reaction monitoring (MRM) was supported with full-scan mass spectrometric confirmation using "information-dependent acquisition" triggered with MRM to "enhanced product ionization" mode of the hybrid quadrupole-ion trap mass analyzer. The LOQ of the test analytes varied between 1 and 10 ng/g with associated recoveries of 80-120% and precision RSD
ISSN:1060-3271
1944-7922
DOI:10.1093/jaoac/94.3.968