Increased myeloperoxidase enzyme activity in plasma is an indicator of inflammation and onset of sepsis

• Published in: Journal of critical care 2011-08, Vol.26 (4), p.435.e1-435.e7
• Main Authors: Kothari, Nikhil, MD, Keshari, Ravi S., MSc, Bogra, Jaishri, MD, Kohli, Monica, MD, Abbas, Haider, MD, Malik, Anita, MD, Dikshit, Madhu, PhD, Barthwal, Manoj K., PhD
• Format: Article
• Language: English
• Subjects: Adult; Biomarkers - blood; Critical Care; Critical Illness; Cytokines; Enzymes; Female; Humans; Immune system; Intensive Care Units; Leukocyte count; Male; Myeloperoxidase; Neutrophils - enzymology; Peroxidase - blood; Plasma; Proteins; Sepsis; Sepsis - enzymology; Shock, Septic - enzymology; SIRS; Statistics, Nonparametric; Systemic Inflammatory Response Syndrome - enzymology
• ISSN: 0883-9441; 1557-8615
• DOI: 10.1016/j.jcrc.2010.09.001

Abstract Introduction Circulating lipopolysaccharides released from bacteria may activate both neutrophils and monocytes. The activated neutrophils release myeloperoxidase (MPO), a specific enzyme with strong oxidative activity. The aim of this study was to evaluate MPO enzyme activity in plasma of critically ill patients and to check the hypothesis that these concentrations in plasma would be higher in sepsis and systemic inflammatory conditions, as neutrophils release their contents before proliferating in response to stress. Material and Methods Blood samples were collected from 105 critically ill patients admitted to the intensive care unit, consisting of those with systemic inflammatory response syndrome (n = 42), sepsis (n = 37), and septic shock (n = 26). Plasma MPO enzyme activity was determined by o -dianisidine-H2 O2 method, modified for 96-well plates. Results The plasma MPO enzyme activity in sepsis patients was significantly higher than that in the control group (mean, 2.4 ± 1.8 in sepsis and 1.86 ± 1.2 nmol per milligram protein per 10 minutes in systemic inflammatory response syndrome vs 0.32 ± 0.11 nmol per milligram protein per 10 minutes in healthy controls). Mean plasma lactate levels in sepsis (7.8 ± 1.2 mmol/L) and shock patients (9.5 ± 1.2 mmol/L) and cytokines like tumor necrosis factor– α , interleukin-8, and interleukin-1 β were simultaneously evaluated to establish onset of inflammation and sepsis. These results show that neutrophil activation occurring during inflammation and sepsis could be detected by plasma MPO concentration. Conclusion The plasma MPO concentrations may be a marker of the neutrophil proliferation and severity of inflammation.