Determination of Fumonisins B1 and B2 in Corn-Based Foods for Infants and Young Children by LC with Immunoaffinity Column Cleanup: Interlaboratory Validation Study

A liquid chromatographic method for the determination of fumonisins B1 (FB1) and B2 (FB2) in corn-based foods for infants and young children was subjected to an interlaboratory validation study involving 11 laboratories. Five blind duplicate sample pairs of each matrix were analyzed to establish the...

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Bibliographic Details
Published in:Journal of AOAC International 2011-05, Vol.94 (3), p.900-908
Main Authors: SOLFRIZZO, Michele, DE GIROLAMO, Annalisa, GAMBACORTA, Lucia, VISCONTI, Angelo, STROKA, Joerg, VAN EGMOND, Hans P
Format: Article
Language:English
Subjects:
Biological and medical sciences
Calibration
Cereal and baking product industries
Child, Preschool
Chromatography - methods
Chromatography, Liquid - methods
Food Analysis - methods
Food Contamination
Food industries
Fumonisins - chemistry
Fundamental and applied biological sciences. Psychology
Humans
Infant
Infant Food - analysis
Laboratories - standards
Reproducibility of Results
Zea mays - chemistry
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Summary:A liquid chromatographic method for the determination of fumonisins B1 (FB1) and B2 (FB2) in corn-based foods for infants and young children was subjected to an interlaboratory validation study involving 11 laboratories. Five blind duplicate sample pairs of each matrix were analyzed to establish the accuracy, repeatability, and reproducibility of the method. Mass fractions in the baby food samples ranged from 89.1 to 384.4 microg/kg FB1 and from 22.5 to 73.6 microg/kg FB2. The method involved a warm extraction with citrate phosphate buffer-methanol-acetonitrile (50 + 25 + 25, v/v/v), a cleanup through an immunoaffinity column, and an end-determination of fumonisins by LC after automated precolumn derivatization with o-phthaldialdehyde reagent. RSDs for within-laboratory repeatability (RSDr) ranged from 6.8 to 23.5% for FB1 and 7.6 to 22.9% for FB2. RSDs for between-laboratory reproducibility (RSDR) ranged from 15.4 to 26.2% for FB1 and 21.6 to 36.3% for FB2. Mean FB1 recoveries from baby foods spiked at 100.0 and 250.0 microg/kg were 89 and 96%, respectively; for FB2 spiked foods at 25.0 and 62.5 microg/kg recoveries were 90 and 85%, respectively. HorRat values ranged from 0.8 to 1.2 for FB1, whereas for FB2 they ranged from 0.9 to 1.4 when calculated according to Horwitz, and from 1.0 to 1.7 when calculated according to Thompson, indicating an acceptable among-laboratory precision for all matrixes (HorRat values
ISSN:1060-3271
1944-7922
DOI:10.1093/jaoac/94.3.900