Molecular imprinting in ionic liquid-modified porous polymer for recognitive separation of three tanshinones from Salvia miltiorrhiza Bunge

• Published in: Analytical and bioanalytical chemistry 2011-03, Vol.399 (7), p.2495-2502
• Main Authors: Tian, Minglei, Bi, Wentao, Row, Kyung Ho
• Format: Article
• Language: English
• Subjects: Adsorption; Analysis; Analytical Chemistry; Beverages; Biochemistry; Characterization and Evaluation of Materials; Chemistry; Chemistry and Materials Science; Chromatographic methods and physical methods associated with chromatography; Chromatography, High Pressure Liquid; Diterpenes, Abietane - isolation & purification; Exact sciences and technology; Food Science; Functional groups; Imidazoles - chemistry; Ionic Liquids; Isotherms; Laboratory Medicine; Mathematical analysis; Methyl alcohol; Molecular imprinting; Molecular Imprinting - methods; Monitoring/Environmental Analysis; Original Paper; Other chromatographic methods; Phenanthrenes - isolation & purification; Plant Extracts - chemistry; Polymer industry; Polymers; Polymers - chemistry; Porosity; Salvia miltiorrhiza; Salvia miltiorrhiza - chemistry; Solid Phase Extraction - methods; Sorbents; Tanshinones
• ISSN: 1618-2642; 1618-2650
• DOI: 10.1007/s00216-010-4641-4

Five ionic liquid-modified porous polymers with different imidazolium-based functional groups were obtained. A molecular imprinting technique was introduced to form the ordered functional groups in the porous structure. The adsorption isotherm was applied to investigate the interactions between the polymers and target compounds: cryptotanshinone; tanshinone I; tanshinone IIA. Thorough comparison revealed that the polymer with a carboxyl group possessed the highest reorganization of the three compounds. After that, the obtained polymer was applied as the sorbent in the solid-phase extraction process to separate the target compounds from methanol extract. The loading volume of extract solution on the sorbent was determined by adsorption isotherm equation and practical test. Under optimized washing and elution conditions, 0.35 mg/g of cryptotanshinone, 0.33 mg/g of tanshinone I, and 0.27 mg/g of tanshinone IIA from plant were obtained by quantitative HPLC analysis. Moreover, six commercial functional drinks containing tanshinones were purified and analyzed.