Quantitative determination of 43 common drugs and drugs of abuse in human serum by HPLC-MS/MS

An analytical procedure for the simultaneous determination in human serum of 43 common drugs of abuse and their metabolites belonging to the different chemical and toxicological classes of amphetamines, benzodiazepines, dibenzazepines, cocaine, lysergic acid diethylamide, opioids, phencyclidine, tri...

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Bibliographic Details
Published in:Analytical and bioanalytical chemistry 2011-04, Vol.400 (1), p.43-50
Main Authors: Bassan, David M, Erdmann, Freidoon, Krüll, Ralf
Format: Article
Language:English
Subjects:
amphetamines
Analysis
Analytical Chemistry
Antidepressants
Benzodiazepines
Biochemistry
blood serum
Characterization and Evaluation of Materials
Chemistry
Chemistry and Materials Science
Chromatography, High Pressure Liquid - methods
cocaine
detection limit
Deuteration
Drug abuse
Drugs
filtration
Food Science
Guidelines
High performance liquid chromatography
Human
humans
Illegal drugs
illicit drugs
Ionization
Laboratory Medicine
LSD (Drug)
Mass spectrometry
Mathematical analysis
metabolites
Monitoring/Environmental Analysis
narcotics
Original Paper
Pharmaceutical Preparations - blood
Precipitation (Meteorology)
quantitative analysis
Serums
Spectrometry, Mass, Electrospray Ionization - methods
Street Drugs - blood
tandem mass spectrometry
Tandem Mass Spectrometry - methods
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Summary:An analytical procedure for the simultaneous determination in human serum of 43 common drugs of abuse and their metabolites belonging to the different chemical and toxicological classes of amphetamines, benzodiazepines, dibenzazepines, cocaine, lysergic acid diethylamide, opioids, phencyclidine, tricyclic antidepressants, and zolpidem, using 33 deuterated standards, is presented. The sample treatment was developed to be a very simple protein precipitation and filtration. All analyses were performed with a high-performance liquid chromatography-electrospray ionization-tandem mass spectrometry in positive ionization mode. All analytes were calibrated up to 550 μg/L. The limit of detection ranged from 0.6 ng/mL (EDDP) to 13.7 ng/mL (flunitrazepam). The method has been validated according to the guidelines of the Gesellschaft für Toxikologische und Forensische Chemie, using three multiple reaction mode (MRM) transitions and retention time for positive compound identification, instead of two MRMs, in anticipation of the new guidelines for January 2011.
ISSN:1618-2642
1618-2650
DOI:10.1007/s00216-011-4784-y