Embryo transfer and sex determination following superovulated hinds inseminated with frozen–thawed sex-sorted Y sperm or unsorted semen in Wapiti ( Cervus elaphus songaricus)

The aim of this study was to evaluate embryo production in superovulated wapiti hinds inseminated with either Y-sorted or unsorted semen. Eighteen hinds were allocated to three treatment groups: AI following multiple ovulation (CIDR/FSH) with 10×10⁶ Y-sorted frozen-thawed semen (Y group, n =6), or 1...

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Bibliographic Details
Published in:Animal reproduction science 2011-07, Vol.126 (3-4), p.245-250
Main Authors: Gao, Q.H, Wang, H.E, Zeng, W.B, Wei, H.J, Han, C.M, Du, H.Z, Zhang, Z.G, Li, X.M
Format: Article
Language:English
Subjects:
Amelogenin gene
Animals
Cervus elaphus canadensis
controlled internal drug release devices
Cryopreservation - veterinary
Deer - embryology
Deer - physiology
DNA
eggs
Embryo
embryo transfer
Embryo Transfer - veterinary
estrus
Female
follicle-stimulating hormone
genes
insemination
Insemination, Artificial - veterinary
Male
oocytes
ovulation
polymerase chain reaction
Polymerase Chain Reaction - veterinary
progeny
semen
Semen - physiology
Semen Analysis - veterinary
Sex determination
Sex Determination Analysis - veterinary
sex ratio
Sex-sorted sperm
spermatozoa
Spermatozoa - physiology
Superovulation
Superovulation - physiology
Wapiti
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Summary:The aim of this study was to evaluate embryo production in superovulated wapiti hinds inseminated with either Y-sorted or unsorted semen. Eighteen hinds were allocated to three treatment groups: AI following multiple ovulation (CIDR/FSH) with 10×10⁶ Y-sorted frozen-thawed semen (Y group, n =6), or 10×10⁶ and 100×10⁶ unsorted frozen-thawed semen for the unsorted (n =6) and the control group (n =6). The embryos from the sixth day following insemination were collected and classified. Fifteen embryos from the unsorted or the control group, and four embryos from the Y group were sex determinated based on DNA analysis of the amelogenin gene. Twenty-one embryos from the Y group and 42 embryos from the unsorted or the control group were transferred into 21 and 42 synchronized recipients via standard procedures on 6th day post estrus, respectively. There were no significant differences in the number of recovered eggs, transferable embryos, degenerated embryos or unfertilized oocytes per hind among the three groups of the control (9.2±3.6, 4.7±1.9, 3.0±2.0, 1.5±1.4), the unsorted (8.2±1.9, 4.8±0.7, 1.7±1.0, 1.7±1.0) and the Y group (8.8±4.2, 4.2±1.8, 2.2±1.2, 2.5±2.1), respectively (P >0.05). The sex ratio of embryos from the Y group (4M/0F) was significantly (P
ISSN:0378-4320
1873-2232
DOI:10.1016/j.anireprosci.2011.05.006