Diversity and Anti-Microbial Activity of Endophytic Actinomycetes Isolated from Medicinal Plants in Panxi Plateau, China

Traditional Chinese medicinal plants are sources of biologically active compounds, providing raw material for pharmaceutical, cosmetic and fragrance industries. The endophytes of medicinal plants participate in biochemical pathways and produce analogous or novel bioactive compounds. Panxi plateau in...

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Bibliographic Details
Published in:Current microbiology 2011-01, Vol.62 (1), p.182-190
Main Authors: Zhao, Ke, Penttinen, Petri, Guan, Tongwei, Xiao, Jing, Chen, Qiang, Xu, Jun, Lindström, Kristina, Zhang, Lili, Zhang, Xiaoping, Strobel, Gary A
Format: Article
Language:English
Subjects:
Actinobacteria - classification
Actinobacteria - genetics
Actinobacteria - isolation & purification
Actinobacteria - metabolism
Actinomycetes
Anti-Infective Agents - metabolism
Bacterial Typing Techniques
Bioactive compounds
Biodiversity
Biomedical and Life Sciences
Biotechnology
China
Cluster Analysis
DNA Fingerprinting
DNA, Bacterial - chemistry
DNA, Bacterial - genetics
DNA, Ribosomal - chemistry
DNA, Ribosomal - genetics
Endophytes
Flowers & plants
Life Sciences
Medicinal plants
Medicine
Microbial activity
Microbiology
Micromonospora
Molecular Sequence Data
Phylogeny
Plant species
Plants, Medicinal - microbiology
Polymorphism, Restriction Fragment Length
Rhodococcus
RNA, Ribosomal, 16S - genetics
Sequence Analysis, DNA
Streptomyces
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Summary:Traditional Chinese medicinal plants are sources of biologically active compounds, providing raw material for pharmaceutical, cosmetic and fragrance industries. The endophytes of medicinal plants participate in biochemical pathways and produce analogous or novel bioactive compounds. Panxi plateau in South-west Sichuan in China with its unique geographical and climatological characteristics is a habitat of a great variety of medicinal plants. In this study, 560 endophytic actinomycetes were isolated from 26 medicinal plant species in Panxi plateau. 60 isolates were selected for 16S rDNA-RFLP analysis and 14 representative strains were chosen for 16S rDNA sequencing. According to the phylogenetic analysis, seven isolates were Streptomyces sp., while the remainder belonged to genera Micromonospora, Oerskovia, Nonomuraea, Promicromonospora and Rhodococcus. Antimicrobial activity analysis combined with the results of amplifying genes coding for polyketide synthetase (PKS-I, PKS-II) and nonribosomal peptide synthetase (NRPS) showed that endophytic actinomycetes isolated from medicinal plants in Panxi plateau had broad-spectrum antimicrobial activity and potential natural product diversity, which further proved that endophytic actinomycetes are valuable reservoirs of novel bioactive compounds.
ISSN:0343-8651
1432-0991
DOI:10.1007/s00284-010-9685-3