A striking common O‐linked N‐acetylglucosaminyl moiety between cruzipain and myosin

Summary Single units of O‐linked N‐acetylglucosamine (GlcNAc), usually components of nuclear and cytoplasmatic proteins, are present at the C‐terminal domain of cruzipain (Cz), a lysosomal major antigen from Trypanosoma cruzi. On the other hand, antibodies directed against some self‐antigens like my...

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Bibliographic Details
Published in:Parasite immunology 2011-07, Vol.33 (7), p.363-370
Main Authors: ACOSTA, D. M., SOPRANO, L. L., FERRERO, M., LANDONI, M., ESTEVA, M. I., COUTO, A. S., DUSCHAK, V. G.
Format: Article
Language:English
Subjects:
Acetylglucosamine - analysis
Acetylglucosamine - immunology
Animals
Antibodies, Protozoan - immunology
Chagas disease
Cross Reactions
cruzipain
Cysteine Endopeptidases - chemistry
Cysteine Endopeptidases - immunology
Enzyme-Linked Immunosorbent Assay
Epitopes - chemistry
Epitopes - immunology
Humans
Mice
Mice, Inbred BALB C
Microscopy, Immunoelectron
Myocardium - pathology
myosin
Myosins - chemistry
Myosins - immunology
O‐N‐acetyl‐d‐glucosamine
Rabbits
Trypanosoma cruzi
Trypanosoma cruzi - chemistry
Trypanosoma cruzi - immunology
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Summary:Summary Single units of O‐linked N‐acetylglucosamine (GlcNAc), usually components of nuclear and cytoplasmatic proteins, are present at the C‐terminal domain of cruzipain (Cz), a lysosomal major antigen from Trypanosoma cruzi. On the other hand, antibodies directed against some self‐antigens like myosin are associated with Chagas heart disease. The participation of O‐GlcNAc moieties in the molecular antigenicity of Cz was determined using GlcNAc linked to aprotinin by ELISA. The immune cross‐reactivity between Cz and myosin is mainly focused in the C‐T domain. ELISA inhibition assays using rabbit sera specific for Cz and C‐T in conjunction with immune‐gold electron microscopy analysis of heart tissues from mice immunized with C‐T confronted with polyclonal rabbit sera specific for Cz and C‐T prior and after myosin adsorption provided evidence which indicates that O‐GlcNAc moieties constitute a common epitope between Cz and either myosin or other cardiac O‐GlcNAc‐containing proteins, showing a new insight into the molecular immune pathogenesis of Chagas heart disease.
ISSN:0141-9838
1365-3024
DOI:10.1111/j.1365-3024.2011.01291.x