Modeling a protein foam fractionation process

• Published in: Applied biochemistry and biotechnology 2000, Vol.84 (1-9), p.1087-1099
• Main Authors: Du, L, Loha, V, Tanner, R.D
• Format: Article
• Language: English
• Subjects: Adsorption; Animals; Biological and medical sciences; Biotechnology; bovine serum albumin; bubbles; Cattle; Chemical Fractionation - instrumentation; Chemical Fractionation - methods; drainage; egg albumen; Foam separation; foams; Fractionation; Fundamental and applied biological sciences. Psychology; Gases; Hydrodynamics; Methods. Procedures. Technologies; Models, Theoretical; Others; Ovalbumin - isolation & purification; Proteins; Proteins - isolation & purification; Serum Albumin, Bovine - isolation & purification; Solutions; sorption isotherms; Various methods and equipments
• ISSN: 0273-2289; 1559-0291; 0273-2289
• DOI: 10.1385/ABAB:84-86:1-9:1087

A simple staged model for the protein foam fractionation process is proposed in this article. This simplified model does not detail the complex foam structure and gas-liquid hydrodynamics in the foam phase but, rather, is built on the conventional theoretical stage concept considering upward bubbles with entrained liquid and downward liquid (drainage) as counter-current flows. To simulate the protein concentration distribution in the liquid along the column by the model, the bubble size and liquid hold-up with respect to the position must be known, as well as the adsorption isotherm of the protein being considered. The model is evaluated for one stage by data from the semibatch foam fractionation of egg albumin and data from the continuous foam fractionation of bovine serum albumin. The effect of two significant variables (superficial gas velocity and feed protein concentration) on enrichment is well predicted by the model, especially for continuous operation and semibatch operation when initial concentration is high.