Mycorrhizal fungi associated with Monotropastrum humile (Ericaceae) in central Japan

We explored the diversity of mycorrhizal fungi associated with Monotropastrum humile in the central part of Japan's main island. We collected 103 M. humile individuals from 12 sites with various forest types. We analyzed the DNA sequences of the internal transcribed spacer region from fungal an...

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Bibliographic Details
Published in:Mycorrhiza 2011-08, Vol.21 (6), p.569-576
Main Authors: Matsuda, Yosuke, Okochi, Shun, Katayama, Tomoyuki, Yamada, Akiyoshi, Ito, Shin-ichiro
Format: Article
Language:English
Subjects:
Agriculture
Biomedical and Life Sciences
Ecology
Ericaceae
Ericaceae - microbiology
Forestry
Fungi - classification
Fungi - genetics
Fungi - isolation & purification
Fungi - physiology
Genetic diversity
Japan
Life Sciences
Microbiology
Molecular Sequence Data
Monotropa uniflora
Mycorrhizae - classification
Mycorrhizae - genetics
Mycorrhizae - isolation & purification
Mycorrhizae - physiology
Original Paper
Phylogeny
Plant Sciences
Roots
Species Specificity
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Summary:We explored the diversity of mycorrhizal fungi associated with Monotropastrum humile in the central part of Japan's main island. We collected 103 M. humile individuals from 12 sites with various forest types. We analyzed the DNA sequences of the internal transcribed spacer region from fungal and plant nuclear ribosomal DNAs to assess the genetic diversity of the fungi associated with M. humile roots and to position the plant with respect to known Monotropoideae groups, respectively. The plants formed a monophyletic clade with other members of M. humile but were separated from M. humile var. glaberrimum and other monotropes (97% bootstrap support). Of the 50 fungal phylotypes, 49 had best matches with the Russulales, and the other had highest similarity with the Thelephoraceae. Our phylogenetic analysis suggests that M. humile roots have a highly specialized association with fungal partners in the Russulaceae. Moreover, a few fungal phylotypes from the M. humile roots had positions neighboring those from Monotropa uniflora roots. These results indicated that the genetic diversity of mycorrhizal fungi of M. humile was highly specific to the Russulaceae, but with high diversity within that family, and that the fungi associated with M. humile differ from those associated with M. uniflora .
ISSN:0940-6360
1432-1890
DOI:10.1007/s00572-011-0365-3