Filamentous fungi’s cell-wall extraction at different stages of ontogenesis and exploration of their carbohydrate composition

Methods of obtaining cell walls (CW) for specimens of mucoraceous molds and ascomycetic affined fungi are developed at the stage of mycelium and resting cells, or spores. CW purity was assessed by electron microscopy, specific staining methods, scourage control, presence of ribose and desoxyribose,...

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Bibliographic Details
Published in:Applied biochemistry and microbiology 2011-07, Vol.47 (4), p.405-411
Main Authors: Andriyanova, D. A., Sergeeva, Ya. E., Kochkina, G. A., Galanina, L. A., Usov, A. I., Feofilova, E. P.
Format: Article
Language:English
Subjects:
Biochemistry
Biomedical and Life Sciences
Carbohydrates
Cellular biology
Chemotaxonomy
Chitin
Fungi
Life Sciences
Medical Microbiology
Microbiology
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Summary:Methods of obtaining cell walls (CW) for specimens of mucoraceous molds and ascomycetic affined fungi are developed at the stage of mycelium and resting cells, or spores. CW purity was assessed by electron microscopy, specific staining methods, scourage control, presence of ribose and desoxyribose, and the comparison of chitin content in whole cells and CW of fungi (a new criteria). The authors discuss the significance of the proposed methods of obtaining pure fractions of CW and of the study of their carbohydrate content for the chemotaxonomy of filamentous fungi.
ISSN:0003-6838
1608-3024
DOI:10.1134/S0003683811040028