Deep sequencing of naupliar-, cyprid- and adult-specific normalised Expressed Sequence Tag (EST) libraries of the acorn barnacle Balanus amphitrite

In order to improve the genetic characterisation of the barnacle Balanus amphitrite, normalised EST libraries for the developmental stages, viz. nauplius (a mix of instars I and II), cyprid and adult, were generated. The libraries were sequenced independently using 454 technologies and 575,666 reads...

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Bibliographic Details
Published in:Biofouling (Chur, Switzerland) Switzerland), 2011-04, Vol.27 (4), p.367-374
Main Authors: De Gregoris, Tristano Bacchetti, Rupp, Oliver, Klages, Sven, Knaust, Florian, Bekel, Thomas, Kube, Michael, Burgess, J. Grant, Arnone, Maria Ina, Goesmann, Alexander, Reinhardt, Richard, Clare, Anthony S.
Format: Article
Language:English
Subjects:
454 sequencing
Adults
Animals
Balanus amphitrite
barnacles
Behavior, Animal
Biofouling
Databases, Genetic
development
Expressed Sequence Tags
Gene Library
Genes
Genetics
Lectins
Libraries
Life Cycle Stages
Marine
Megabalanus rosa
Nucleotides
Sequence Analysis, DNA
settlement
Settlements
Thoracica - genetics
Thoracica - growth & development
User-Computer Interface
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Summary:In order to improve the genetic characterisation of the barnacle Balanus amphitrite, normalised EST libraries for the developmental stages, viz. nauplius (a mix of instars I and II), cyprid and adult, were generated. The libraries were sequenced independently using 454 technologies and 575,666 reads were generated. For adults, 4843 unique isotigs were estimated and 6754 and 7506 in the cyprid and naupliar stage, respectively. It was found that some of the previously proposed cyprid-specific bcs genes were also expressed during the naupliar and adult stage. Furthermore, as lectins have been hypothesised to influence settlement cue recognition in barnacles, the database was searched for lectin-like isotigs. Two proteins, uniquely expressed in either the cyprid or the adult stage, matched a mannose receptor, and their nucleotide sequences were 33% and 31% identical to a lectin (BRA-3) isolated from Megabalanus rosa. Further characterisation of these genes may suggest their involvement in settlement.
ISSN:0892-7014
1029-2454
DOI:10.1080/08927014.2011.577211