In vivo bioactivity of DNA-based coatings: An experimental study in rats

DNA‐based coatings possess beneficial properties useful for medical and dental implants. The present study evaluated the potential in vivo bioactivity of DNA‐based coatings, either or not pretreated in simulated body fluid (SBF). DNA‐based coatings were generated on titanium cylinders using layer‐by...

Full description

Saved in:
Bibliographic Details
Published in:Journal of biomedical materials research. Part A 2010-03, Vol.92A (3), p.931-941
Main Authors: Schouten, Corinne, van den Beucken, Jeroen J. J. P., Meijer, Gert J., Sommerdijk, Nico A. J. M., Spauwen, Paul H. M., Jansen, John A.
Format: Article
Language:English
Subjects:
(animal) rat study
Animals
bioactivity
Biocompatible Materials
Biological and medical sciences
Calcium Phosphates
Crystallography, X-Ray
DNA
DNA-coating
layer-by-layer deposition
Medical sciences
Microscopy, Electron, Scanning
Prostheses and Implants
Rats
SBF
Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases
Technology. Biomaterials. Equipments
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:DNA‐based coatings possess beneficial properties useful for medical and dental implants. The present study evaluated the potential in vivo bioactivity of DNA‐based coatings, either or not pretreated in simulated body fluid (SBF). DNA‐based coatings were generated on titanium cylinders using layer‐by‐layer deposition, with bis‐ureido‐surfactant as the cationic component and DNA as the anionic component. Noncoated titanium implants and CaP‐coated implants served as controls. A total of 80 implants, divided in four experimental groups (n = 10) were implanted unilaterally into the lateral femoral condyles of 80 rats. After implantation periods of 1 and 4 weeks, the bone‐to‐implant contact and bone volume around the implants were determined histomorphometrically. The results of this study showed that DNA‐based coatings and CaP coatings increased bone‐to‐implant contact after 1 week compared to noncoated controls. After 4 weeks of implantation, bone‐to‐implant contact increased significantly for SBF pretreated DNA coatings and CaP coatings, whereas DNA‐coated implants showed no additional effect. The bone‐to‐implant contact of noncoated controls increased to the level of the DNA‐coated implants. Consequently, this study demonstrates that DNA‐based coatings are histocompatible and favor early bone responses. SBF‐pretreated DNA‐based coatings were found to increase both early and late peri‐implant bone responses. © 2009 Wiley Periodicals, Inc. J Biomed Mater Res, 2010
ISSN:1549-3296
1552-4965
1552-4965
DOI:10.1002/jbm.a.32446