Phenotypic and functional differentiation of KG-1 into dendritic-like cells

Abstract The cell line KG-1 has been used as an in vitro model for human dendritic cell (DC) differentiation. We have investigated the response of KG-1 cells to stimulation with a number of factors known to induce differentiation and/or maturation of DCs in vitro . KG-1 cells showed no differentiati...

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Bibliographic Details
Published in:Immunobiology (1979) 2008-01, Vol.213 (1), p.75-86
Main Authors: Teobald, I, Dunnion, D.J, Whitbread, M, Curnow, S.J, Browning, M.J
Format: Article
Language:English
Subjects:
Advanced Basic Science
Allergy and Immunology
Antigen presentation
CD40 Ligand - immunology
CD40 Ligand - metabolism
Cell Adhesion
Cell Differentiation
Cell Line
Cell Proliferation
CpG Islands
Cytokines - immunology
Cytokines - metabolism
Dendritic cell
Dendritic Cells - cytology
Dendritic Cells - immunology
Dendritic Cells - metabolism
Differentiation
Humans
KG-1
Lymphocyte Culture Test, Mixed
Oligodeoxyribonucleotides - immunology
Pinocytosis
T-Lymphocytes - immunology
T-Lymphocytes - metabolism
Tetradecanoylphorbol Acetate - pharmacology
Tumor Necrosis Factor-alpha - immunology
Tumor Necrosis Factor-alpha - metabolism
Vaccine
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Summary:Abstract The cell line KG-1 has been used as an in vitro model for human dendritic cell (DC) differentiation. We have investigated the response of KG-1 cells to stimulation with a number of factors known to induce differentiation and/or maturation of DCs in vitro . KG-1 cells showed no differentiation in response to LPS, CpG oligodeoxynucleotide or CD40 ligation. Culture in the presence of TNF- α induced some differentiation, but only treatment with PMA and ionomycin (with or without prior culture in GM-CSF and IL-4) induced morphological and phenotypic changes consistent with DC-like maturation, and even these maximally differentiated KG-1 cells showed lower levels of surface marker expression, macromolecular endocytosis, and ability to stimulate in allogeneic MLR compared with in vitro monocyte-derived DCs. Our data show that KG-1 cells differentiate in vitro into cells with DC-like functional characteristics under the influence of strong inducers of cellular activation, but lack the potency of mature DCs in key aspects of professional antigen presenting cells.
ISSN:0171-2985
1878-3279
DOI:10.1016/j.imbio.2007.06.001