Genetic structure and diversity of Phakopsora pachyrhizi isolates from soyabean

Simple sequence repeat (SSR) markers were used to classify 116 isolates of Phakopsora pachyrhizi, the cause of soyabean rust, collected from infected soyabean leaves in four agroecological zones in Nigeria. A high degree of genetic variation was observed within the sampled populations of P. pachyrhi...

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Bibliographic Details
Published in:Plant pathology 2011-08, Vol.60 (4), p.719-729
Main Authors: Twizeyimana, M, Ojiambo, P.S, Haudenshield, J.S, Caetano-Anollés, G, Pedley, K.F, Bandyopadhyay, R, Hartman, G.L
Format: Article
Language:English
Subjects:
agroecological zones
Asexual reproduction
Biological and medical sciences
Differentiation
Dispersal
Evolution
Fundamental and applied biological sciences. Psychology
Fungal plant pathogens
Genetic diversity
genetic markers
Genetic structure
genetic variation
genotype
Genotypes
Glycine max
Leaves
Mutation
Phakopsora pachyrhizi
Phylogeny
Phytopathology. Animal pests. Plant and forest protection
plant pathogenic fungi
population biology
Population differentiation
population genetics
Rust
rust diseases
simple sequence length polymorphism
Simple sequence repeats
soyabean
soyabean rust
soybeans
SSR genotype
virulence phenotype
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Summary:Simple sequence repeat (SSR) markers were used to classify 116 isolates of Phakopsora pachyrhizi, the cause of soyabean rust, collected from infected soyabean leaves in four agroecological zones in Nigeria. A high degree of genetic variation was observed within the sampled populations of P. pachyrhizi. Eighty-four distinct genotypes were identified among three of the four agroecological zones. Nei's average genetic diversity across geographical regions was 0·22. Hierarchical analysis of molecular variance showed low genetic differentiation among all populations of P. pachyrhizi. The majority (> 90%) of the genetic diversity was distributed within each soyabean field, while approximately 6% of the genetic diversity was distributed among fields within geographic regions. Low population differentiation was indicated by the low FST values among populations, suggesting a wide dispersal of identical genotypes on a regional scale. Phylogenetic analysis indicated a strictly clonal structure of the populations and five main groups were observed, with group II accounting for 30% of the entire population. Because of the asexual reproduction of P. pachyrhizi, single-step mutations in SSR genotypes are likely to account for the genetic differences within each group.
ISSN:0032-0862
1365-3059
DOI:10.1111/j.1365-3059.2011.02428.x