effect of carbon source on the secretome of Kluyveromyces lactis

A proteomic analysis was performed on spent fermentation medium following bioreactor propagation of a wild-type industrial strain to identify proteins naturally secreted by Kluyveromyces lactis cells. Here, we report changes detected in the K. lactis secretome as a result of growth in three differen...

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Bibliographic Details
Published in:Proteomics (Weinheim) 2009-10, Vol.9 (20), p.4744-4754
Main Authors: Madinger, Catherine L, Sharma, Shamik S, Anton, Brian P, Fields, Lauren G, Cushing, Michelle L, Canovas, Julie, Taron, Christopher H, Benner, Jack S
Format: Article
Language:English
Subjects:
Carbon - metabolism
Computational Biology
Fungal Proteins - analysis
Fungal Proteins - metabolism
Glycosylation
Kluyveromyces - chemistry
Kluyveromyces - growth & development
Kluyveromyces - metabolism
Kluyveromyces lactis
Multi-dimensional
NanoESI-MS/MS
Nanoproteomics
Proteome - analysis
Proteome - metabolism
Secretome
Sugar variation
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Summary:A proteomic analysis was performed on spent fermentation medium following bioreactor propagation of a wild-type industrial strain to identify proteins naturally secreted by Kluyveromyces lactis cells. Here, we report changes detected in the K. lactis secretome as a result of growth in three different carbon sources: glucose, galactose and glycerol. A total of 151 secreted proteins were detected by multi-dimensional separations and reversed-phase online nanoESI-MS/MS analysis. From these, we were able to identify 63 proteins (termed the "base secretome") that were common to all three fermentation conditions. The majority of base secretome proteins, 79%, possessed general secretory pathway (GSP) sequences and were involved with cell wall structure, glycosylation, carbohydrate metabolism and proteolysis. There was little variation in the functional groupings of base secretome GSP proteins and GSP proteins that were not part of the base secretome. In contrast, the majority of non-GSP proteins detected were not part of the base secretome and the functions of these proteins varied significantly. Finally, through further identification of non-GSP proteins in carbon sources not originally tested, we have gained further evidence of a protein export mechanism separate from the GSP in K. lactis.
ISSN:1615-9853
1615-9861
1615-9861
DOI:10.1002/pmic.200800915