Decolorization of azo dyes by Shewanella oneidensis MR-1 in the presence of humic acids

• Published in: Applied microbiology and biotechnology 2011-07, Vol.91 (2), p.417-424
• Main Authors: Liu, Guangfei, Zhou, Jiti, Wang, Jing, Wang, Xiujuan, Jin, Ruofei, Lv, Hong
• Format: Article
• Language: English
• Subjects: Acids; Amaranth Dye; Anaerobic conditions; Analysis; Azo Compounds - chemistry; Azo Compounds - metabolism; Azo dyes; Bacteria; Biodegradation, Environmental; Biological and medical sciences; Biomedical and Life Sciences; Bioremediation; Biotechnology; Chemicals; Coloring Agents - chemistry; Coloring Agents - metabolism; Dyes; Environmental Biotechnology; Fundamental and applied biological sciences. Psychology; Humic acids; Humic Substances; Industrial Microbiology - methods; Industrial wastewater; Laboratories; Life Sciences; Microbial Genetics and Genomics; Microbiology; Microorganisms; Shewanella - classification; Shewanella - metabolism; Shewanella oneidensis; Sludge; Studies; Water Pollutants, Chemical - metabolism
• ISSN: 0175-7598; 1432-0614
• DOI: 10.1007/s00253-011-3273-8

The effects of humic acid (HA) on azo dye decolorization by Shewanella oneidensis MR-1 were studied. It was found that HA species isolated from different sources could all accelerate the decolorization of Acid Red 27 (AR27). Anoxic and anaerobic conditions were required for the enhancement of azo dye decolorization by HA. In the presence of 50 mg DOC L −1 Aldrich HA, 15–29% increases in decolorization efficiencies of azo dyes with different structures were achieved in 11 h. The enhancing effects increased with the increase of HA concentrations ranging from 25 to 150 mg DOC L −1 , and the decolorization rates were directly proportional to the HA concentrations when they were below 100 mg DOC L −1 . Lactate and formate were good electron donors for AR27 decolorization in the presence of HA. Both nitrate (0.1–3.0 mM) and nitrite (0.3–1.2 mM) inhibited AR27 decolorization in the presence of HA, and negligible decolorization was observed before their removal. Soluble FeCl 3 could accelerate the decolorization process in the presence of HA, whereas insoluble hematite could not. These findings may affect the understanding of bioremediation of azo dye-polluted environments and help improve the treatment of azo dye wastewaters.