IDENTIFICATION OF COENZYME Q₁₀ FROM PORPHYRIDIUM PURPUREUM (RHODOPHYTA) BY MATRIX-ASSISTED LASER DESORPTION IONIZATION CURVED FIELD REFLECTRON MASS SPECTROMETRY

Antioxidant agents from natural sources are currently the focus of scientific interest and are part of several natural product screenings. Coenzymes Q (CoQ, ubiquinones) are integral parts of the electron transport chain of the inner mitochondrial membrane. As antioxidants they protect phospholipids...

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Bibliographic Details
Published in:Journal of phycology 2011-06, Vol.47 (3), p.687-691
Main Authors: Klein, Barbara C, Bartel, Sebastian J, Darsow, Kai H, Naumann, Ivonne, Walter, Christian, Buchholz, Rainer, Lange, Harald A
Format: Article
Language:English
Subjects:
antioxidant
antioxidants
coenzyme Q10
coenzymes
desorption
electron transport chain
Escherichia coli
humans
ionization
MALDI curved field reflectron mass spectrometer
mass spectrometry
peroxidation
Porphyridium
Porphyridium purpureum
Rhodophyta
Saccharomyces cerevisiae
spectrometers
ubiquinones
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Summary:Antioxidant agents from natural sources are currently the focus of scientific interest and are part of several natural product screenings. Coenzymes Q (CoQ, ubiquinones) are integral parts of the electron transport chain of the inner mitochondrial membrane. As antioxidants they protect phospholipids against peroxidation and are also involved in various processes of tissue protection. Their natural occurrence was validated for Saccharomyces cerevisiae as CoQ₆, for Escherichia coli as CoQ₈, and for humans as CoQ₁₀. After carrying out a preparative reversed-phase (RP)-HPLC separation of extracts isolated from unicellular red alga Porphyridium purpureum (Bory) K. M. Drew et R. Ross, it was possible to identify a 2,3-dimethoxy-5-methyl-6-decaprenyl-1,4-benzoquinone (CoQ₁₀) within these extracts using a matrix-assisted laser desorption ionization (MALDI) curved field reflectron (CFR) mass spectrometer. Detected mass fragments showed a high significance and could be structurally interpreted for both commercialized standard and CoQ₁₀ isolated from P. purpureum.
ISSN:0022-3646
1529-8817
DOI:10.1111/j.1529-8817.2011.00978.x