POTEH hypomethylation, a new epigenetic biomarker for glioma prognosis

Abstract POTE ankyrin domain family, member H (POTEH) belongs to POTE family, which expresses in many cancers. In this study, methylation status of POTEH promoter and its correlation with clinicopathological parameters were evaluated in glioma tissues and cells. Bisulfite sequencing PCR was carried...

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Bibliographic Details
Published in:Brain research 2011-05, Vol.1391, p.125-131
Main Authors: Liu, Xiaoping, Tang, Hailin, Zhang, Zuping, Li, Wenjuan, Wang, Zeyou, Zheng, Ying, Wu, Minghua, Li, Guiyuan
Format: Article
Language:English
Subjects:
Adolescent
Adult
Antigens, Neoplasm - metabolism
Biological and medical sciences
Biomarkers - metabolism
Brain Neoplasms - diagnosis
Brain Neoplasms - genetics
Cell Line, Tumor
Child
Child, Preschool
DNA Methylation - genetics
Epigenomics
Female
Follow-Up Studies
Glioma
Glioma - diagnosis
Glioma - genetics
Humans
Hypomethylation
Male
Medical sciences
Middle Aged
Neurology
POTEH
Prognosis
Proteins - genetics
Proteins - metabolism
Tumors of the nervous system. Phacomatoses
Young Adult
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Summary:Abstract POTE ankyrin domain family, member H (POTEH) belongs to POTE family, which expresses in many cancers. In this study, methylation status of POTEH promoter and its correlation with clinicopathological parameters were evaluated in glioma tissues and cells. Bisulfite sequencing PCR was carried out to investigate the promoter methylation status of POTEH in tumor of 96 glioma patients and glioma cells U251, SF767, and SF126. The effect of promoter hypomethylation on protein expression was evaluated by immunohistochemistry. POTEH was hypomethylated in 81.3% gliomas and none in normal brain tissues, and correlated significantly with its protein expression. But there was no remarkable relationship between sex, age, advanced tumor grade and POTEH hypomethylation. With the grade progressing, POTEH protein expression was enhanced. The correlation between POTEH hypomethylation, protein expression and overall survival was statistically significant. In POTEH hypomethylation group, patients with POTEH high expression had shorter overall survival than those with low expression. Hypomethylation of POTEH promoter in gliomas accounted for POTEH protein overexpression and poor outcome in a subset of patients. Detection of these epigenetic changes in tumors may provide information regarding prognosis.
ISSN:0006-8993
1872-6240
DOI:10.1016/j.brainres.2011.03.042