Resistance mechanism of Leptochloa chinensis Nees to propanil

The resistance mechanism of Leptochloa chinensis Nees to propanil was investigated, based on propanil metabolism, aryl acylamidase activity, and chlorophyll fluorescence at the 8 week growth stage of L. chinensis. The concentration of propanil in the leaf and culm extracts of the resistant (R) and s...

Full description

Saved in:
Bibliographic Details
Published in:Weed biology and management 2011-06, Vol.11 (2), p.57-63
Main Authors: SAHID, ISMAIL B., KARSO, JULIANA, CHUAH, TSE-SENG
Format: Article
Language:English
Subjects:
3,4-Dichloroaniline
aryl acylamidase
Biotypes
Chlorophyll
chlorophyll fluorescence
Fluorescence
Gas chromatography
Growth stage
Leaves
Leptochloa
Leptochloa chinensis (L.) Nees
Metabolism
Photosystem II
propanil
Weeds
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The resistance mechanism of Leptochloa chinensis Nees to propanil was investigated, based on propanil metabolism, aryl acylamidase activity, and chlorophyll fluorescence at the 8 week growth stage of L. chinensis. The concentration of propanil in the leaf and culm extracts of the resistant (R) and susceptible (S) biotypes, as measured by gas chromatography (GC), was found to increase after propanil treatment. The concentration of propanil in the leaf and culm extracts of the S biotype at 72 h was 1.55 and 0.49 µg mL−1, respectively. However, a lower concentration of propanil was observed in the R biotype, as compared to that in the S biotype. The residue of 3,4‐dichloroaniline, as measured by GC, was detected only in the leaf extracts of the R biotype. In contrast, no residue of 3,4‐dichloroaniline was observed in the S biotype. The level of aryl acylamidase in the leaf tissue extracts of the R biotype was ∼140% higher than that in the S biotype. The fluorescence studies showed that propanil inhibited the quantum efficiency of the photosystem II in both the R and S biotypes after 2 h of incubation time. However, when the leaf disks were transferred and incubated in deionized water for 48 h, the quantum efficiency increased in the R biotype but decreased in the S biotype. These results suggest that propanil metabolism, enhanced by aryl acylamidase activity, is the most likely factor contributing towards the mechanism of propanil resistance in L. chinensis plants at the 8 week growth stage.
ISSN:1444-6162
1445-6664
DOI:10.1111/j.1445-6664.2011.00405.x