The expression of bone matrix proteins induced by different bioimplants in a rabbit sinus lift model

• Published in: Journal of biomedical materials research. Part A 2010-12, Vol.95A (4), p.1048-1054
• Main Authors: Hu, ZhenMing, Peel, Sean A.F., Ho, Stephen K. C., Sándor, George K. B., Su, Yan, Clokie, Cameron M. L.
• Format: Article
• Language: English
• Subjects: angiogenesis; Animals; Biocompatibility; Biocompatible Materials - pharmacology; bioimplant; Biological and medical sciences; Biomedical materials; BMP (bone morphogenetic proteins); bone healing; Bone Matrix - drug effects; Bone Matrix - metabolism; Bone Matrix - pathology; bone matrix proteins; Bones; Cattle; Collagen Type I - metabolism; Extracellular Matrix Proteins - metabolism; Grafting; immunochemistry; Immunohistochemistry; Implants, Experimental; Integrin-Binding Sialoprotein - metabolism; Male; Maxillary Sinus - drug effects; Maxillary Sinus - metabolism; Maxillary Sinus - pathology; Medical sciences; Models, Animal; Osteocalcin - metabolism; Proteins; rabbit; Rabbits; sinus; Sinuses; Staining; Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases; Technology. Biomaterials. Equipments
• ISSN: 1549-3296; 1552-4965; 1552-4965
• DOI: 10.1002/jbm.a.32911

This study aimed to analyze the expression of bone matrix proteins and CD31 by immunohistochemistry after maxillary sinus grafting with different bioimplants in a rabbit model. Rabbit demineralized bone matrix (DBM), partially purified bovine bone morphogenetic proteins (BMP), a mixture of BMP with DBM (BMP/DBM), or particulated autogenous bone was grafted into the maxillary sinuses of 42 rabbits. Animals were sacrificed at 2 and 8 weeks. Immunohistochemistry was used to investigate the expression of type 1 collagen (COL1), osteonectin (ON), osteocalcin (OC), bone sialoprotein (BSP), osteopontin (OPN), and CD31. Sinuses grafted with BMP were filled with trabeculae of woven bone that was strongly immunoreactive for COL1, OC, ON, and BSP. BMP/DBM showed strongly positive immunoreactivity for these proteins within the newly formed bone, but weak immunoreactivity in the DBM particles. Immunoreactivity for COL1, OC, ON, and BSP in DBM sinuses was only seen in the osteoblasts rimming the grafted bone particles. The staining of autogenous bone graft sinuses was similar to those grafted with DBM. OPN staining was detected in autogenous bone graft, BMP/DBM, and BMP bioimplants. CD31 staining was strongest in BMP and BMP/noncollagenous matrix proteins sinuses. These results suggest that exogenous BMP enhances not only osteogenesis but also angiogenesis, an important part of bone repair. © 2010 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2010.