Selective targeting of green fluorescent nanodiamond conjugates to mitochondria in HeLa cells

Fluorescent cellular biomarkers play a prominent role in biosciences. Most of the available biomarkers have some drawbacks due to either physical and optical or cytotoxic properties. In view of this, we investigated the potential of green fluorescent nanodiamonds as biomarkers in living cells. Nanod...

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Bibliographic Details
Published in:Journal of biophotonics 2009-10, Vol.2 (10), p.596-606
Main Authors: Mkandawire, Msaukiranji, Pohl, Andrea, Gubarevich, Tatiana, Lapina, Victoria, Appelhans, Dietmar, Rödel, Gerhard, Pompe, Wolfgang, Schreiber, Jürgen, Opitz, Jörg
Format: Article
Language:English
Subjects:
Actins - immunology
antibodies
Antibodies, Monoclonal - chemistry
Antibodies, Monoclonal - immunology
biofunctionalization
Carbodiimides - chemistry
Dendrimers - chemistry
Diamond - analysis
Diamond - chemistry
fluorescent biomarkers
Fluorescent Dyes - analysis
Fluorescent Dyes - chemistry
glycodendrimers
HeLa Cells
Humans
Immunoconjugates - chemistry
Immunoconjugates - metabolism
Liposomes - chemistry
Microscopy, Fluorescence
Mitochondria - metabolism
Mitochondrial Proteins - immunology
Molecular Probe Techniques
Nanoparticles - analysis
Nanoparticles - chemistry
Particle Size
Protamines - chemistry
Spectrometry, Fluorescence
Spectrophotometry, Infrared
Static Electricity
Succinimides - chemistry
Surface Properties
transfection
UDD immunoconjugates
ultradispersed diamonds (UDD)
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Summary:Fluorescent cellular biomarkers play a prominent role in biosciences. Most of the available biomarkers have some drawbacks due to either physical and optical or cytotoxic properties. In view of this, we investigated the potential of green fluorescent nanodiamonds as biomarkers in living cells. Nanodiamonds were functionalized by attaching antibodies that target intracellular structures such as actin filaments and mitochondria. Then, the nanodiamond conjugates were transfected into HeLa cells. Transfections were mediated by 4th‐generation dendrimers, cationic liposomes and protamine sulfate. Using fluorescence microscopy, we confirmed successful transfections of the nanodiamonds into HeLa cells. Nanodiamond fluorescence could be easily differentiated from cellular autofluorescence. Furthermore, nanodiamonds could be targeted selectively to intracellular structures. Therefore, nanodiamonds are a promising tool for intracellular assays. (© 2009 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
ISSN:1864-063X
1864-0648
1864-0648
DOI:10.1002/jbio.200910002