Demasculinization of male fish by wastewater treatment plant effluent

• Published in: Aquatic toxicology 2011-06, Vol.103 (3), p.213-221
• Main Authors: Vajda, Alan M., Barber, Larry B., Gray, James L., Lopez, Elena M., Bolden, Ashley M., Schoenfuss, Heiko L., Norris, David O.
• Format: Article
• Language: English
• Subjects: Agnatha. Pisces; Animal, plant and microbial ecology; Animals; Applied ecology; Benzhydryl Compounds; Biological and medical sciences; Catostomus commersoni; Colorado; Disorders of Sex Development - chemically induced; Ecotoxicology, biological effects of pollution; Endocrine disruption; Endocrine Disruptors - analysis; Endocrine Disruptors - toxicity; Environmental Monitoring; Estradiol - analysis; Estradiol - toxicity; Estrogen; Estrogens - analysis; Estrogens - toxicity; Estrone - analysis; Estrone - toxicity; Ethinyl Estradiol - analysis; Ethinyl Estradiol - toxicity; Fish; Fishes - metabolism; Fishes - physiology; Freshwater; Fundamental and applied biological sciences. Psychology; General aspects; Male; Nonylphenol; Phenols - analysis; Phenols - toxicity; Pimephales promelas; Sex Ratio; Sperm Count; Testis - drug effects; Vertebrates: general zoology, morphology, phylogeny, systematics, cytogenetics, geographical distribution; Vitellogenins - metabolism; Waste Disposal, Fluid; Wastewater; Water Pollutants, Chemical - analysis; Water Pollutants, Chemical - toxicity
• ISSN: 0166-445X; 1879-1514
• DOI: 10.1016/j.aquatox.2011.02.007

Adult male fathead minnows ( Pimephales promelas) were exposed to effluent from the City of Boulder, Colorado wastewater treatment plant (WWTP) under controlled conditions in the field to determine if the effluent induced reproductive disruption in fish. Gonadal intersex and other evidence of reproductive disruption were previously identified in white suckers ( Catostomus commersoni) in Boulder Creek downstream from this WWTP effluent outfall. Fish were exposed within a mobile flow-through exposure laboratory in July 2005 and August 2006 to WWTP effluent (EFF), Boulder Creek water (REF), or mixtures of EFF and REF for up to 28 days. Primary (sperm abundance) and secondary (nuptial tubercles and dorsal fat pads) sex characteristics were demasculinized within 14 days of exposure to 50% and 100% EFF. Vitellogenin was maximally elevated in both 50% and 100% EFF treatments within 7 days and significantly elevated by 25% EFF within 14 days. The steroidal estrogens 17β-estradiol, estrone, estriol, and 17α-ethynylestradiol, as well as estrogenic alkylphenols and bisphenol A were identified within the EFF treatments and not in the REF treatment. These results support the hypothesis that the reproductive disruption observed in this watershed is due to endocrine-active chemicals in the WWTP effluent.