Transfection efficiency for size-separated cells synchronized in cell cycle by microfluidic device

Non-viral system generally demonstrates less efficacious in transgene delivery than viral system; however it represents a safer alternative to viral system. In this study, transfection efficiency for human hepatocellular liver carcinoma cells synchronized in cell cycle at G0/G1 phase, which was sort...

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Bibliographic Details
Published in:Biomedical microdevices 2011-08, Vol.13 (4), p.725-729
Main Authors: Migita, Satoshi, Hanagata, Nobutaka, Tsuya, Daiju, Yamazaki, Tomohiko, Sugimoto, Yoshimasa, Ikoma, Toshiyuki
Format: Article
Language:English
Subjects:
Biological and Medical Physics
Biomedical engineering
Biomedical Engineering and Bioengineering
Biophysics
Cell Adhesion
Cell cycle
Cell Separation
Engineering
Engineering Fluid Dynamics
Equipment Design
Fluid dynamics
G1 Phase - genetics
Gene Expression
Green Fluorescent Proteins - genetics
Green Fluorescent Proteins - metabolism
Hep G2 Cells
Humans
Hydrodynamics
Microfluidic Analytical Techniques - instrumentation
Microfluidic Analytical Techniques - methods
Nanotechnology
Plasmids
Resting Phase, Cell Cycle - genetics
Transfection - methods
Transgenes
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Summary:Non-viral system generally demonstrates less efficacious in transgene delivery than viral system; however it represents a safer alternative to viral system. In this study, transfection efficiency for human hepatocellular liver carcinoma cells synchronized in cell cycle at G0/G1 phase, which was sorted in size with a microfluidic device based on hydrodynamic filtration, was investigated by using a reverse transfection method. The synchronized cells were recovered at the yield of 80% from the micro-channel, and green fluorescent protein gene encoding plasmid mixed with lipofectoamine was transfected. The transfection efficiency of the cells at G0/G1 phase was 1.8 times higher than non-synchronized cells. The manipulation of cell cycle status could increase transfection efficiency in non-viral system, indicating size-based cell cycle synchronization is a powerful tool as a noninvasive method for bioscience and biotechnology.
ISSN:1387-2176
1572-8781
DOI:10.1007/s10544-011-9542-6