Rhodnius prolixus: Identification of immune-related genes up-regulated in response to pathogens and parasites using suppressive subtractive hybridization

We report the identification of immune-related molecules from the fat body, and intestine of Rhodnius prolixus, an important vector of Chagas disease. Insects were challenged by introducing pathogens or Trypanosoma cruzi, the parasite that causes Chagas disease, into the hemocoel. RNA from intestine...

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Bibliographic Details
Published in:Developmental & Comparative Immunology 2007, Vol.31 (2), p.109-120
Main Authors: Ursic-Bedoya, Raul J., Lowenberger, Carl A.
Format: Article
Language:English
Subjects:
Animals
Antimicrobial Cationic Peptides - genetics
Chagas Disease - transmission
Expressed Sequence Tags
Fat Body - metabolism
Gene Expression Regulation
Innate immunity
Intestines - metabolism
Mucins - genetics
Nitric Oxide - physiology
Nucleic Acid Hybridization - methods
Parasitic infection
Rhodnius - immunology
Rhodnius - microbiology
Rhodnius - parasitology
Rhodnius prolixus
Suppressive subtractive hybridization
Transferrin - genetics
Trypanosoma cruzi
Up-Regulation
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Summary:We report the identification of immune-related molecules from the fat body, and intestine of Rhodnius prolixus, an important vector of Chagas disease. Insects were challenged by introducing pathogens or Trypanosoma cruzi, the parasite that causes Chagas disease, into the hemocoel. RNA from intestines, or fat body were isolated 24 h after stimulation. We used suppressive subtractive hybridization to identify immune-related genes, generated three subtracted libraries, sequenced the clones and assembled the sequences. The functional annotation revealed expressed sequence tags (ESTs) generated in response to various stimuli in all tissues, and included pathogen recognition molecules, regulatory molecules, and effector molecules.
ISSN:0145-305X
1879-0089
1365-2567
DOI:10.1016/j.dci.2006.05.008