Loading…

Rhodnius prolixus: Identification of immune-related genes up-regulated in response to pathogens and parasites using suppressive subtractive hybridization

We report the identification of immune-related molecules from the fat body, and intestine of Rhodnius prolixus, an important vector of Chagas disease. Insects were challenged by introducing pathogens or Trypanosoma cruzi, the parasite that causes Chagas disease, into the hemocoel. RNA from intestine...

Full description

Saved in:

Bibliographic Details
Published in:	Developmental & Comparative Immunology 2007, Vol.31 (2), p.109-120
Main Authors:	Ursic-Bedoya, Raul J., Lowenberger, Carl A.
Format:	Article
Language:	English
Subjects:	Animals Antimicrobial Cationic Peptides - genetics Chagas Disease - transmission Expressed Sequence Tags Fat Body - metabolism Gene Expression Regulation Innate immunity Intestines - metabolism Mucins - genetics Nitric Oxide - physiology Nucleic Acid Hybridization - methods Parasitic infection Rhodnius - immunology Rhodnius - microbiology Rhodnius - parasitology Rhodnius prolixus Suppressive subtractive hybridization Transferrin - genetics Trypanosoma cruzi Up-Regulation
Citations:	Items that this one cites Items that cite this one
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

cited_by	cdi_FETCH-LOGICAL-c414t-88bba21f393b1d739f47a7bc9d290ce16545e04bda84fd03ede1bd7787f458af3
cites	cdi_FETCH-LOGICAL-c414t-88bba21f393b1d739f47a7bc9d290ce16545e04bda84fd03ede1bd7787f458af3
container_end_page	120
container_issue	2
container_start_page	109
container_title	Developmental & Comparative Immunology
container_volume	31
creator	Ursic-Bedoya, Raul J. Lowenberger, Carl A.
description	We report the identification of immune-related molecules from the fat body, and intestine of Rhodnius prolixus, an important vector of Chagas disease. Insects were challenged by introducing pathogens or Trypanosoma cruzi, the parasite that causes Chagas disease, into the hemocoel. RNA from intestines, or fat body were isolated 24 h after stimulation. We used suppressive subtractive hybridization to identify immune-related genes, generated three subtracted libraries, sequenced the clones and assembled the sequences. The functional annotation revealed expressed sequence tags (ESTs) generated in response to various stimuli in all tissues, and included pathogen recognition molecules, regulatory molecules, and effector molecules.
doi_str_mv	10.1016/j.dci.2006.05.008
format	article
fullrecord	<record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_883029190</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0145305X06000851</els_id><sourcerecordid>20734858</sourcerecordid><originalsourceid>FETCH-LOGICAL-c414t-88bba21f393b1d739f47a7bc9d290ce16545e04bda84fd03ede1bd7787f458af3</originalsourceid><addsrcrecordid>eNqFkc2OFCEUhYnROO3oA7gxrHRVJVRBAboyE38mmcTEaOKOUHCrm041lEBNHN_Et5W2O3E3suFw851Dcg9CzylpKaHD633rrG87QoaW8JYQ-QBtqBSqqVI9RBtCGW96wr9foCc570k9kpLH6IIOsmNciQ36_WUXXfBrxkuKs_-55jf42kEofvLWFB8DjhP2h8MaoEkwmwIObyFAxutSB9v1NPIBJ8hLDBlwiXgxZRcrlrEJrr6Syb4cPdmHLc7rslQ6-1uoeizJ2HLUu7sxeed__f33KXo0mTnDs_N9ib59eP_16lNz8_nj9dW7m8Yyykoj5Tiajk696kfqRK8mJowYrXKdIhbowBkHwkZnJJsc6cEBHZ0QUkyMSzP1l-jVKbcu4McKueiDzxbm2QSIa9ZS9qRTVJFKvryXHGTPB9Gp_4IdET2TXFaQnkCbYs4JJr0kfzDpTlOijxXrva4V62PFmnBd-6ueF-fwdTyA--c4d1qBtycA6tZuPSSdrYdgwfkEtmgX_T3xfwA9Zrvn</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>20734858</pqid></control><display><type>article</type><title>Rhodnius prolixus: Identification of immune-related genes up-regulated in response to pathogens and parasites using suppressive subtractive hybridization</title><source>ScienceDirect Freedom Collection 2022-2024</source><source>Wiley</source><source>PubMed Central</source><creator>Ursic-Bedoya, Raul J. ; Lowenberger, Carl A.</creator><creatorcontrib>Ursic-Bedoya, Raul J. ; Lowenberger, Carl A.</creatorcontrib><description>We report the identification of immune-related molecules from the fat body, and intestine of Rhodnius prolixus, an important vector of Chagas disease. Insects were challenged by introducing pathogens or Trypanosoma cruzi, the parasite that causes Chagas disease, into the hemocoel. RNA from intestines, or fat body were isolated 24 h after stimulation. We used suppressive subtractive hybridization to identify immune-related genes, generated three subtracted libraries, sequenced the clones and assembled the sequences. The functional annotation revealed expressed sequence tags (ESTs) generated in response to various stimuli in all tissues, and included pathogen recognition molecules, regulatory molecules, and effector molecules.</description><identifier>ISSN: 0145-305X</identifier><identifier>EISSN: 1879-0089</identifier><identifier>EISSN: 1365-2567</identifier><identifier>DOI: 10.1016/j.dci.2006.05.008</identifier><identifier>PMID: 16824597</identifier><language>eng</language><publisher>United States: Elsevier Ltd</publisher><subject>Animals ; Antimicrobial Cationic Peptides - genetics ; Chagas Disease - transmission ; Expressed Sequence Tags ; Fat Body - metabolism ; Gene Expression Regulation ; Innate immunity ; Intestines - metabolism ; Mucins - genetics ; Nitric Oxide - physiology ; Nucleic Acid Hybridization - methods ; Parasitic infection ; Rhodnius - immunology ; Rhodnius - microbiology ; Rhodnius - parasitology ; Rhodnius prolixus ; Suppressive subtractive hybridization ; Transferrin - genetics ; Trypanosoma cruzi ; Up-Regulation</subject><ispartof>Developmental & Comparative Immunology, 2007, Vol.31 (2), p.109-120</ispartof><rights>2006 Elsevier Ltd</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c414t-88bba21f393b1d739f47a7bc9d290ce16545e04bda84fd03ede1bd7787f458af3</citedby><cites>FETCH-LOGICAL-c414t-88bba21f393b1d739f47a7bc9d290ce16545e04bda84fd03ede1bd7787f458af3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,4024,27923,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16824597$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ursic-Bedoya, Raul J.</creatorcontrib><creatorcontrib>Lowenberger, Carl A.</creatorcontrib><title>Rhodnius prolixus: Identification of immune-related genes up-regulated in response to pathogens and parasites using suppressive subtractive hybridization</title><title>Developmental & Comparative Immunology</title><addtitle>Dev Comp Immunol</addtitle><description>We report the identification of immune-related molecules from the fat body, and intestine of Rhodnius prolixus, an important vector of Chagas disease. Insects were challenged by introducing pathogens or Trypanosoma cruzi, the parasite that causes Chagas disease, into the hemocoel. RNA from intestines, or fat body were isolated 24 h after stimulation. We used suppressive subtractive hybridization to identify immune-related genes, generated three subtracted libraries, sequenced the clones and assembled the sequences. The functional annotation revealed expressed sequence tags (ESTs) generated in response to various stimuli in all tissues, and included pathogen recognition molecules, regulatory molecules, and effector molecules.</description><subject>Animals</subject><subject>Antimicrobial Cationic Peptides - genetics</subject><subject>Chagas Disease - transmission</subject><subject>Expressed Sequence Tags</subject><subject>Fat Body - metabolism</subject><subject>Gene Expression Regulation</subject><subject>Innate immunity</subject><subject>Intestines - metabolism</subject><subject>Mucins - genetics</subject><subject>Nitric Oxide - physiology</subject><subject>Nucleic Acid Hybridization - methods</subject><subject>Parasitic infection</subject><subject>Rhodnius - immunology</subject><subject>Rhodnius - microbiology</subject><subject>Rhodnius - parasitology</subject><subject>Rhodnius prolixus</subject><subject>Suppressive subtractive hybridization</subject><subject>Transferrin - genetics</subject><subject>Trypanosoma cruzi</subject><subject>Up-Regulation</subject><issn>0145-305X</issn><issn>1879-0089</issn><issn>1365-2567</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><recordid>eNqFkc2OFCEUhYnROO3oA7gxrHRVJVRBAboyE38mmcTEaOKOUHCrm041lEBNHN_Et5W2O3E3suFw851Dcg9CzylpKaHD633rrG87QoaW8JYQ-QBtqBSqqVI9RBtCGW96wr9foCc570k9kpLH6IIOsmNciQ36_WUXXfBrxkuKs_-55jf42kEofvLWFB8DjhP2h8MaoEkwmwIObyFAxutSB9v1NPIBJ8hLDBlwiXgxZRcrlrEJrr6Syb4cPdmHLc7rslQ6-1uoeizJ2HLUu7sxeed__f33KXo0mTnDs_N9ib59eP_16lNz8_nj9dW7m8Yyykoj5Tiajk696kfqRK8mJowYrXKdIhbowBkHwkZnJJsc6cEBHZ0QUkyMSzP1l-jVKbcu4McKueiDzxbm2QSIa9ZS9qRTVJFKvryXHGTPB9Gp_4IdET2TXFaQnkCbYs4JJr0kfzDpTlOijxXrva4V62PFmnBd-6ueF-fwdTyA--c4d1qBtycA6tZuPSSdrYdgwfkEtmgX_T3xfwA9Zrvn</recordid><startdate>2007</startdate><enddate>2007</enddate><creator>Ursic-Bedoya, Raul J.</creator><creator>Lowenberger, Carl A.</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SS</scope><scope>7T5</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>C1K</scope><scope>F1W</scope><scope>H95</scope><scope>H97</scope><scope>L.G</scope></search><sort><creationdate>2007</creationdate><title>Rhodnius prolixus: Identification of immune-related genes up-regulated in response to pathogens and parasites using suppressive subtractive hybridization</title><author>Ursic-Bedoya, Raul J. ; Lowenberger, Carl A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c414t-88bba21f393b1d739f47a7bc9d290ce16545e04bda84fd03ede1bd7787f458af3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Animals</topic><topic>Antimicrobial Cationic Peptides - genetics</topic><topic>Chagas Disease - transmission</topic><topic>Expressed Sequence Tags</topic><topic>Fat Body - metabolism</topic><topic>Gene Expression Regulation</topic><topic>Innate immunity</topic><topic>Intestines - metabolism</topic><topic>Mucins - genetics</topic><topic>Nitric Oxide - physiology</topic><topic>Nucleic Acid Hybridization - methods</topic><topic>Parasitic infection</topic><topic>Rhodnius - immunology</topic><topic>Rhodnius - microbiology</topic><topic>Rhodnius - parasitology</topic><topic>Rhodnius prolixus</topic><topic>Suppressive subtractive hybridization</topic><topic>Transferrin - genetics</topic><topic>Trypanosoma cruzi</topic><topic>Up-Regulation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ursic-Bedoya, Raul J.</creatorcontrib><creatorcontrib>Lowenberger, Carl A.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 3: Aquatic Pollution & Environmental Quality</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><jtitle>Developmental & Comparative Immunology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ursic-Bedoya, Raul J.</au><au>Lowenberger, Carl A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Rhodnius prolixus: Identification of immune-related genes up-regulated in response to pathogens and parasites using suppressive subtractive hybridization</atitle><jtitle>Developmental & Comparative Immunology</jtitle><addtitle>Dev Comp Immunol</addtitle><date>2007</date><risdate>2007</risdate><volume>31</volume><issue>2</issue><spage>109</spage><epage>120</epage><pages>109-120</pages><issn>0145-305X</issn><eissn>1879-0089</eissn><eissn>1365-2567</eissn><abstract>We report the identification of immune-related molecules from the fat body, and intestine of Rhodnius prolixus, an important vector of Chagas disease. Insects were challenged by introducing pathogens or Trypanosoma cruzi, the parasite that causes Chagas disease, into the hemocoel. RNA from intestines, or fat body were isolated 24 h after stimulation. We used suppressive subtractive hybridization to identify immune-related genes, generated three subtracted libraries, sequenced the clones and assembled the sequences. The functional annotation revealed expressed sequence tags (ESTs) generated in response to various stimuli in all tissues, and included pathogen recognition molecules, regulatory molecules, and effector molecules.</abstract><cop>United States</cop><pub>Elsevier Ltd</pub><pmid>16824597</pmid><doi>10.1016/j.dci.2006.05.008</doi><tpages>12</tpages></addata></record>
fulltext	fulltext
identifier	ISSN: 0145-305X
ispartof	Developmental & Comparative Immunology, 2007, Vol.31 (2), p.109-120
issn	0145-305X 1879-0089 1365-2567
language	eng
recordid	cdi_proquest_miscellaneous_883029190
source	ScienceDirect Freedom Collection 2022-2024; Wiley; PubMed Central
subjects	Animals Antimicrobial Cationic Peptides - genetics Chagas Disease - transmission Expressed Sequence Tags Fat Body - metabolism Gene Expression Regulation Innate immunity Intestines - metabolism Mucins - genetics Nitric Oxide - physiology Nucleic Acid Hybridization - methods Parasitic infection Rhodnius - immunology Rhodnius - microbiology Rhodnius - parasitology Rhodnius prolixus Suppressive subtractive hybridization Transferrin - genetics Trypanosoma cruzi Up-Regulation
title	Rhodnius prolixus: Identification of immune-related genes up-regulated in response to pathogens and parasites using suppressive subtractive hybridization
url	http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-29T19%3A31%3A07IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Rhodnius%20prolixus:%20Identification%20of%20immune-related%20genes%20up-regulated%20in%20response%20to%20pathogens%20and%20parasites%20using%20suppressive%20subtractive%20hybridization&rft.jtitle=Developmental%20&%20Comparative%20Immunology&rft.au=Ursic-Bedoya,%20Raul%20J.&rft.date=2007&rft.volume=31&rft.issue=2&rft.spage=109&rft.epage=120&rft.pages=109-120&rft.issn=0145-305X&rft.eissn=1879-0089&rft_id=info:doi/10.1016/j.dci.2006.05.008&rft_dat=%3Cproquest_cross%3E20734858%3C/proquest_cross%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c414t-88bba21f393b1d739f47a7bc9d290ce16545e04bda84fd03ede1bd7787f458af3%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=20734858&rft_id=info:pmid/16824597&rfr_iscdi=true