Structure and function of the symbiosis partners of the lung lichen (Lobaria pulmonaria L. Hoffm.) analyzed by metaproteomics

Environmental proteomics, also referred to as metaproteomics, is an emerging technology to study the structure and function of microbial communities. Here, we applied semi‐quantitative label‐free proteomics using one‐dimensional gel electrophoresis combined with LC‐MS/MS and normalized spectral coun...

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Published in:Proteomics (Weinheim) 2011-07, Vol.11 (13), p.2752-2756
Main Authors: Schneider, Thomas, Schmid, Emanuel, de Castro Jr, João V., Cardinale, Massimiliano, Eberl, Leo, Grube, Martin, Berg, Gabriele, Riedel, Kathrin
Format: Article
Language:English
Subjects:
Algae
Analytical, structural and metabolic biochemistry
Aquatic plants
Archaea
Bacteria - metabolism
Bacteria - ultrastructure
Bacterial proteins
Biological and medical sciences
Electrophoresis, Polyacrylamide Gel - methods
Fundamental and applied biological sciences. Psychology
Fungi - metabolism
Fungi - ultrastructure
Imaging, Three-Dimensional
Lichens
Lichens - microbiology
Lichens - ultrastructure
Lobaria pulmonaria
Metaproteomics
Microbial activity
Microbial ecology
Microbiology
Miscellaneous
Proteins
Proteobacteria
Proteomics
Proteomics - methods
Symbiosis
Symbiosis - physiology
Tandem Mass Spectrometry - methods
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Summary:Environmental proteomics, also referred to as metaproteomics, is an emerging technology to study the structure and function of microbial communities. Here, we applied semi‐quantitative label‐free proteomics using one‐dimensional gel electrophoresis combined with LC‐MS/MS and normalized spectral counting together with fluorescence in situ hybridization and confocal laser scanning microscopy to characterize the metaproteome of the lung lichen symbiosis Lobaria pulmonaria. In addition to the myco‐ and photobiont, L. pulmonaria harbors proteins from a highly diverse prokaryotic community, which is dominated by Proteobacteria and including also Archaea. While fungal proteins are most dominant (75.4% of all assigned spectra), about the same amount of spectra were assigned to prokaryotic proteins (10%) and to the green algal photobiont (9%). While the latter proteins were found to be mainly associated with energy and carbohydrate metabolism, a major proportion of fungal and bacterial proteins appeared to be involved in PTMs and protein turnover and other diverse functions.
ISSN:1615-9853
1615-9861
1615-9861
DOI:10.1002/pmic.201000679