Proteomic analysis of the cold stress response in the moss, Physcomitrella patens

Cold stress has adverse effects on plant growth and development. Plants respond and acclimate to cold stress through various biochemical and physiological processes, thereby acquiring stress tolerance. To better understand the basis for tolerance, we carried out a proteomic study in the model moss,...

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Bibliographic Details
Published in:Proteomics (Weinheim) 2009-10, Vol.9 (19), p.4529-4538
Main Authors: Wang, Xiaoqin, Yang, Pingfang, Zhang, Xiaofeng, Xu, Yinong, Kuang, Tingyun, Shen, Shihua, He, Yikun
Format: Article
Language:English
Subjects:
2‐DE
Analytical, structural and metabolic biochemistry
Biological and medical sciences
Bryopsida - chemistry
Bryopsida - physiology
Cell Membrane - metabolism
Chromatography, Liquid
Cold stress
Cold Temperature
Electrophoresis, Gel, Two-Dimensional
Fundamental and applied biological sciences. Psychology
Gene Expression Profiling
LC‐MS/MS
Lipid Metabolism
Lipids - analysis
Miscellaneous
Molecular Sequence Data
Physcomitrella
Physcomitrella patens
Plant Proteins - analysis
Plant Proteins - genetics
Plant proteomics
Proteins
Proteome
Proteome - analysis
Signal Transduction - physiology
Stress, Physiological
Tandem Mass Spectrometry
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Summary:Cold stress has adverse effects on plant growth and development. Plants respond and acclimate to cold stress through various biochemical and physiological processes, thereby acquiring stress tolerance. To better understand the basis for tolerance, we carried out a proteomic study in the model moss, Physcomitrella patens, characterizing gametophore proteins with 2-DE and mass spectroscopy. Following exposure to 0°C for up to 3 days, out of the more than 1000 protein spots reproducibly resolved, only 45 changed in abundance by at least 1.5-fold. Of these, 35 were identified by tryptic digestion and mass spectroscopy. Photosynthetic proteins decreased, whereas many catabolic proteins increased. In addition, cold stress up-regulated a variety of signaling, cytoskeleton, and defense proteins and few proteins in these classes were down-regulated. Up-regulated proteins include the 14-3-3-like protein, actin, HSP70s, lipoxygenases, and cytochrome P450 proteins. These results point to pathways that are important for the mechanism of cold stress response in P. patens and by extension to the entire plant kingdom.
ISSN:1615-9853
1615-9861
1615-9861
DOI:10.1002/pmic.200900062