A novel biomimetic composite scaffold hybridized with mesenchymal stem cells in repair of rat bone defects models

• Published in: Journal of biomedical materials research. Part A 2010-11, Vol.95A (2), p.495-503
• Main Authors: Xu, Caixia, Su, Peiqiang, Wang, Yingjun, Chen, Xiaofeng, Meng, Yongchun, Liu, Chang, Yu, Xinbing, Yang, Xuhui, Yu, Weihua, Zhang, Xiuming, Xiang, Andy Peng
• Format: Article
• Language: English
• Subjects: Animals; Biocompatibility; Biocompatible Materials - chemistry; Biocompatible Materials - metabolism; bioglass-collagen-hyaluronic acid-phosphatidylserine; Biological and medical sciences; Biomedical materials; Biomimetics; Biotechnology; Bone Regeneration - physiology; bone tissue engineering; Bones; Cells, Cultured; Ceramics - chemistry; Collagen - chemistry; composite scaffold; Defects; Female; Femur - pathology; Fundamental and applied biological sciences. Psychology; Health. Pharmaceutical industry; Hyaluronic Acid - chemistry; Implantation; Industrial applications and implications. Economical aspects; Materials Testing; Medical sciences; Mesenchymal Stem Cell Transplantation - methods; mesenchymal stem cells; Mesenchymal Stromal Cells - cytology; Mesenchymal Stromal Cells - physiology; Miscellaneous; Rats; Rats, Sprague-Dawley; Scaffolds; Stem cells; Stress, Mechanical; Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases; Surgical implants; Technology. Biomaterials. Equipments; Tissue Scaffolds - chemistry
• ISSN: 1549-3296; 1552-4965; 1552-4965
• DOI: 10.1002/jbm.a.32877

In this study, the in vivo bone‐regenerative potential of a novel bioactive glass‐collagen‐hyaluronic acid‐Phosphatidylserine (BG‐COL‐HYA‐PS) composite scaffold hybridized with mesenchymal stem cells (MSCs) was investigated in a rat bone defect model. HrGFP‐labeled MSCs were cultured for 2 weeks on the BG‐COL‐HYA‐PS scaffold before implantation into the defect. A cell‐free scaffold and an untreated defect were used as controls. The regeneration process was evaluated by histology, X‐ray, and mechanical rigidity experiments at different time points post‐implantation. The results revealed that BG‐COL‐HYA‐PS scaffold exhibited a low inflammatory response and foreign body response within 3 weeks. At week 6, those responses disappeared following the resorption of scaffolds and the formation of new bone. Compared with the pure scaffold or empty group, the introduction of MSCs into the porous scaffold dramatically enhanced the efficiency of the new bone formation and biomechanical property of the femur. In addition, the transplanted MSCs could survive for up to 3 weeks or longer. The results demonstrated that the BG‐COL‐HYA‐PS scaffold was biocompatible and osteoconductive and the transplanted MSCs with the scaffold enhanced the healing of the bone defect. © 2010 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2010.