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Dorfin ameliorates phenotypes in a transgenic mouse model of amyotrophic lateral sclerosis
Amyotrophic lateral sclerosis (ALS) is a fatal neurodegenerative disease that is characterized by progressive motor neuron degeneration and leads to death within a few years of diagnosis. One of the pathogenic mechanisms of ALS is proposed to be a dysfunction in the protein quality‐control machinery...
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Published in: | Journal of neuroscience research 2010-01, Vol.88 (1), p.123-135 |
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creator | Sone, Jun Niwa, Jun-ichi Kawai, Kaori Ishigaki, Shinsuke Yamada, Shin-ichi Adachi, Hiroaki Katsuno, Masahisa Tanaka, Fumiaki Doyu, Manabu Sobue, Gen |
description | Amyotrophic lateral sclerosis (ALS) is a fatal neurodegenerative disease that is characterized by progressive motor neuron degeneration and leads to death within a few years of diagnosis. One of the pathogenic mechanisms of ALS is proposed to be a dysfunction in the protein quality‐control machinery. Dorfin has been identified as a ubiquitin ligase (E3) that recognizes and ubiquitinates mutant SOD1 proteins, thereby accelerating their degradation and reducing their cellular toxicity. We examined the effects of human Dorfin overexpression in G93A mutant SOD1 transgenic mice, a mouse model of familial ALS. In addition to causing a decrease in the amount of mutant SOD1 protein in the spinal cord, Dorfin overexpression ameliorated neurological phenotypes and motor neuron degeneration. Our results indicate that Dorfin overexpression or the activation or induction of E3 may be a therapeutic avenue for mutant SOD1‐associated ALS. © 2009 Wiley‐Liss, Inc. |
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One of the pathogenic mechanisms of ALS is proposed to be a dysfunction in the protein quality‐control machinery. Dorfin has been identified as a ubiquitin ligase (E3) that recognizes and ubiquitinates mutant SOD1 proteins, thereby accelerating their degradation and reducing their cellular toxicity. We examined the effects of human Dorfin overexpression in G93A mutant SOD1 transgenic mice, a mouse model of familial ALS. In addition to causing a decrease in the amount of mutant SOD1 protein in the spinal cord, Dorfin overexpression ameliorated neurological phenotypes and motor neuron degeneration. Our results indicate that Dorfin overexpression or the activation or induction of E3 may be a therapeutic avenue for mutant SOD1‐associated ALS. © 2009 Wiley‐Liss, Inc.</description><identifier>ISSN: 0360-4012</identifier><identifier>ISSN: 1097-4547</identifier><identifier>EISSN: 1097-4547</identifier><identifier>DOI: 10.1002/jnr.22175</identifier><identifier>PMID: 19610091</identifier><language>eng</language><publisher>Hoboken: Wiley Subscription Services, Inc., A Wiley Company</publisher><subject>ALS ; Amyotrophic Lateral Sclerosis - genetics ; Amyotrophic Lateral Sclerosis - metabolism ; Amyotrophic Lateral Sclerosis - pathology ; Amyotrophic Lateral Sclerosis - physiopathology ; Analysis of Variance ; Animals ; Astrocytes - metabolism ; Astrocytes - pathology ; Blotting, Western ; Disease Models, Animal ; Dorfin ; G93A mutant SOD1 ; Gene Dosage - genetics ; Immunohistochemistry ; Immunoprecipitation ; Kaplan-Meier Estimate ; Mice ; Mice, Transgenic ; Motor Skills - physiology ; Nerve Degeneration - genetics ; Nerve Degeneration - metabolism ; Nerve Degeneration - pathology ; neurodegeneration ; Neurons - metabolism ; Neurons - pathology ; Phenotype ; Rotarod Performance Test ; Spinal Cord - metabolism ; Spinal Cord - pathology ; Spinal Cord - physiopathology ; Spinal Nerve Roots - metabolism ; Spinal Nerve Roots - pathology ; Spinal Nerve Roots - physiopathology ; Superoxide Dismutase - genetics ; Superoxide Dismutase - metabolism ; ubiquitin ligase ; Ubiquitin-Protein Ligases - genetics ; Ubiquitin-Protein Ligases - metabolism</subject><ispartof>Journal of neuroscience research, 2010-01, Vol.88 (1), p.123-135</ispartof><rights>Copyright © 2009 Wiley‐Liss, Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4925-f7bcc9baeca494fa04f2709bb0fc8d96577094ddd5d28dc11bbf7ea257dd51043</citedby><cites>FETCH-LOGICAL-c4925-f7bcc9baeca494fa04f2709bb0fc8d96577094ddd5d28dc11bbf7ea257dd51043</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19610091$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sone, Jun</creatorcontrib><creatorcontrib>Niwa, Jun-ichi</creatorcontrib><creatorcontrib>Kawai, Kaori</creatorcontrib><creatorcontrib>Ishigaki, Shinsuke</creatorcontrib><creatorcontrib>Yamada, Shin-ichi</creatorcontrib><creatorcontrib>Adachi, Hiroaki</creatorcontrib><creatorcontrib>Katsuno, Masahisa</creatorcontrib><creatorcontrib>Tanaka, Fumiaki</creatorcontrib><creatorcontrib>Doyu, Manabu</creatorcontrib><creatorcontrib>Sobue, Gen</creatorcontrib><title>Dorfin ameliorates phenotypes in a transgenic mouse model of amyotrophic lateral sclerosis</title><title>Journal of neuroscience research</title><addtitle>J. Neurosci. Res</addtitle><description>Amyotrophic lateral sclerosis (ALS) is a fatal neurodegenerative disease that is characterized by progressive motor neuron degeneration and leads to death within a few years of diagnosis. One of the pathogenic mechanisms of ALS is proposed to be a dysfunction in the protein quality‐control machinery. Dorfin has been identified as a ubiquitin ligase (E3) that recognizes and ubiquitinates mutant SOD1 proteins, thereby accelerating their degradation and reducing their cellular toxicity. We examined the effects of human Dorfin overexpression in G93A mutant SOD1 transgenic mice, a mouse model of familial ALS. In addition to causing a decrease in the amount of mutant SOD1 protein in the spinal cord, Dorfin overexpression ameliorated neurological phenotypes and motor neuron degeneration. Our results indicate that Dorfin overexpression or the activation or induction of E3 may be a therapeutic avenue for mutant SOD1‐associated ALS. © 2009 Wiley‐Liss, Inc.</description><subject>ALS</subject><subject>Amyotrophic Lateral Sclerosis - genetics</subject><subject>Amyotrophic Lateral Sclerosis - metabolism</subject><subject>Amyotrophic Lateral Sclerosis - pathology</subject><subject>Amyotrophic Lateral Sclerosis - physiopathology</subject><subject>Analysis of Variance</subject><subject>Animals</subject><subject>Astrocytes - metabolism</subject><subject>Astrocytes - pathology</subject><subject>Blotting, Western</subject><subject>Disease Models, Animal</subject><subject>Dorfin</subject><subject>G93A mutant SOD1</subject><subject>Gene Dosage - genetics</subject><subject>Immunohistochemistry</subject><subject>Immunoprecipitation</subject><subject>Kaplan-Meier Estimate</subject><subject>Mice</subject><subject>Mice, Transgenic</subject><subject>Motor Skills - physiology</subject><subject>Nerve Degeneration - genetics</subject><subject>Nerve Degeneration - metabolism</subject><subject>Nerve Degeneration - pathology</subject><subject>neurodegeneration</subject><subject>Neurons - metabolism</subject><subject>Neurons - pathology</subject><subject>Phenotype</subject><subject>Rotarod Performance Test</subject><subject>Spinal Cord - metabolism</subject><subject>Spinal Cord - pathology</subject><subject>Spinal Cord - physiopathology</subject><subject>Spinal Nerve Roots - metabolism</subject><subject>Spinal Nerve Roots - pathology</subject><subject>Spinal Nerve Roots - physiopathology</subject><subject>Superoxide Dismutase - genetics</subject><subject>Superoxide Dismutase - metabolism</subject><subject>ubiquitin ligase</subject><subject>Ubiquitin-Protein Ligases - genetics</subject><subject>Ubiquitin-Protein Ligases - metabolism</subject><issn>0360-4012</issn><issn>1097-4547</issn><issn>1097-4547</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><recordid>eNqFkE9P3DAQxS1UBMvCgS9Q5VZxCNiOHcfHdmkpK_5UVSsQF8txxiXUiYOdFd1vj2EXekJcxjOe33uyH0L7BB8SjOnRXR8OKSWCb6AJwVLkjDPxAU1wUeKcYUK30U6MdxhjKXmxhbaJLJNQkgm6OfbBtn2mO3CtD3qEmA230PtxOaT2aZONQffxD_StyTq_iJBqAy7zNqmWfgx-uE0rl7RBuywaB8HHNu6iTatdhL31OUW_v339Nfuen12enM4-n-WGScpzK2pjZK3BaCaZ1ZhZKrCsa2xN1ciSizSxpml4Q6vGEFLXVoCmXKQrglkxRZ9WvkPw9wuIo-raaMA53UN6rqqqAhdUEP4-WUpeSVKWiTxYkSZ9JQawaghtp8NSEayeMlcpc_WceWI_rl0XdQfNf3IdcgKOVsBD62D5tpOaX_x8scxXijaO8O9VocNfVYoikVcXJ2rOfpwXs_JafSkeARn6nBI</recordid><startdate>201001</startdate><enddate>201001</enddate><creator>Sone, Jun</creator><creator>Niwa, Jun-ichi</creator><creator>Kawai, Kaori</creator><creator>Ishigaki, Shinsuke</creator><creator>Yamada, Shin-ichi</creator><creator>Adachi, Hiroaki</creator><creator>Katsuno, Masahisa</creator><creator>Tanaka, Fumiaki</creator><creator>Doyu, Manabu</creator><creator>Sobue, Gen</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>201001</creationdate><title>Dorfin ameliorates phenotypes in a transgenic mouse model of amyotrophic lateral sclerosis</title><author>Sone, Jun ; Niwa, Jun-ichi ; Kawai, Kaori ; Ishigaki, Shinsuke ; Yamada, Shin-ichi ; Adachi, Hiroaki ; Katsuno, Masahisa ; Tanaka, Fumiaki ; Doyu, Manabu ; Sobue, Gen</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4925-f7bcc9baeca494fa04f2709bb0fc8d96577094ddd5d28dc11bbf7ea257dd51043</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>ALS</topic><topic>Amyotrophic Lateral Sclerosis - genetics</topic><topic>Amyotrophic Lateral Sclerosis - metabolism</topic><topic>Amyotrophic Lateral Sclerosis - pathology</topic><topic>Amyotrophic Lateral Sclerosis - physiopathology</topic><topic>Analysis of Variance</topic><topic>Animals</topic><topic>Astrocytes - metabolism</topic><topic>Astrocytes - pathology</topic><topic>Blotting, Western</topic><topic>Disease Models, Animal</topic><topic>Dorfin</topic><topic>G93A mutant SOD1</topic><topic>Gene Dosage - genetics</topic><topic>Immunohistochemistry</topic><topic>Immunoprecipitation</topic><topic>Kaplan-Meier Estimate</topic><topic>Mice</topic><topic>Mice, Transgenic</topic><topic>Motor Skills - physiology</topic><topic>Nerve Degeneration - genetics</topic><topic>Nerve Degeneration - metabolism</topic><topic>Nerve Degeneration - pathology</topic><topic>neurodegeneration</topic><topic>Neurons - metabolism</topic><topic>Neurons - pathology</topic><topic>Phenotype</topic><topic>Rotarod Performance Test</topic><topic>Spinal Cord - metabolism</topic><topic>Spinal Cord - pathology</topic><topic>Spinal Cord - physiopathology</topic><topic>Spinal Nerve Roots - metabolism</topic><topic>Spinal Nerve Roots - pathology</topic><topic>Spinal Nerve Roots - physiopathology</topic><topic>Superoxide Dismutase - genetics</topic><topic>Superoxide Dismutase - metabolism</topic><topic>ubiquitin ligase</topic><topic>Ubiquitin-Protein Ligases - genetics</topic><topic>Ubiquitin-Protein Ligases - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sone, Jun</creatorcontrib><creatorcontrib>Niwa, Jun-ichi</creatorcontrib><creatorcontrib>Kawai, Kaori</creatorcontrib><creatorcontrib>Ishigaki, Shinsuke</creatorcontrib><creatorcontrib>Yamada, Shin-ichi</creatorcontrib><creatorcontrib>Adachi, Hiroaki</creatorcontrib><creatorcontrib>Katsuno, Masahisa</creatorcontrib><creatorcontrib>Tanaka, Fumiaki</creatorcontrib><creatorcontrib>Doyu, Manabu</creatorcontrib><creatorcontrib>Sobue, Gen</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>Journal of neuroscience research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sone, Jun</au><au>Niwa, Jun-ichi</au><au>Kawai, Kaori</au><au>Ishigaki, Shinsuke</au><au>Yamada, Shin-ichi</au><au>Adachi, Hiroaki</au><au>Katsuno, Masahisa</au><au>Tanaka, Fumiaki</au><au>Doyu, Manabu</au><au>Sobue, Gen</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Dorfin ameliorates phenotypes in a transgenic mouse model of amyotrophic lateral sclerosis</atitle><jtitle>Journal of neuroscience research</jtitle><addtitle>J. Neurosci. Res</addtitle><date>2010-01</date><risdate>2010</risdate><volume>88</volume><issue>1</issue><spage>123</spage><epage>135</epage><pages>123-135</pages><issn>0360-4012</issn><issn>1097-4547</issn><eissn>1097-4547</eissn><abstract>Amyotrophic lateral sclerosis (ALS) is a fatal neurodegenerative disease that is characterized by progressive motor neuron degeneration and leads to death within a few years of diagnosis. One of the pathogenic mechanisms of ALS is proposed to be a dysfunction in the protein quality‐control machinery. Dorfin has been identified as a ubiquitin ligase (E3) that recognizes and ubiquitinates mutant SOD1 proteins, thereby accelerating their degradation and reducing their cellular toxicity. We examined the effects of human Dorfin overexpression in G93A mutant SOD1 transgenic mice, a mouse model of familial ALS. In addition to causing a decrease in the amount of mutant SOD1 protein in the spinal cord, Dorfin overexpression ameliorated neurological phenotypes and motor neuron degeneration. Our results indicate that Dorfin overexpression or the activation or induction of E3 may be a therapeutic avenue for mutant SOD1‐associated ALS. © 2009 Wiley‐Liss, Inc.</abstract><cop>Hoboken</cop><pub>Wiley Subscription Services, Inc., A Wiley Company</pub><pmid>19610091</pmid><doi>10.1002/jnr.22175</doi><tpages>13</tpages></addata></record> |
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subjects | ALS Amyotrophic Lateral Sclerosis - genetics Amyotrophic Lateral Sclerosis - metabolism Amyotrophic Lateral Sclerosis - pathology Amyotrophic Lateral Sclerosis - physiopathology Analysis of Variance Animals Astrocytes - metabolism Astrocytes - pathology Blotting, Western Disease Models, Animal Dorfin G93A mutant SOD1 Gene Dosage - genetics Immunohistochemistry Immunoprecipitation Kaplan-Meier Estimate Mice Mice, Transgenic Motor Skills - physiology Nerve Degeneration - genetics Nerve Degeneration - metabolism Nerve Degeneration - pathology neurodegeneration Neurons - metabolism Neurons - pathology Phenotype Rotarod Performance Test Spinal Cord - metabolism Spinal Cord - pathology Spinal Cord - physiopathology Spinal Nerve Roots - metabolism Spinal Nerve Roots - pathology Spinal Nerve Roots - physiopathology Superoxide Dismutase - genetics Superoxide Dismutase - metabolism ubiquitin ligase Ubiquitin-Protein Ligases - genetics Ubiquitin-Protein Ligases - metabolism |
title | Dorfin ameliorates phenotypes in a transgenic mouse model of amyotrophic lateral sclerosis |
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