Cultured autologous bone marrow stem cells inhibit bony fusion in a rabbit model of posterolateral lumbar fusion with autologous bone graft

Abstract Mesenchymal stem cells (MSCs) have been isolated from various tissues and expanded in culture. MSCs add osteogenic potential to ceramic scaffolds when used together. A spinal fusion rabbit model was used to evaluate whether a pellet of cultured, autologous bone marrow MSCs (BMSCs) with oste...

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Bibliographic Details
Published in:Journal of clinical neuroscience 2010-04, Vol.17 (4), p.481-485
Main Authors: Urrutia, Julio, Mery, Pablo, Martínez, Rafael, Pizarro, Felipe, Apablaza, Daniel, Mardones, Rodrigo
Format: Article
Language:English
Subjects:
Animal model
Animals
Autografts
Bone grafts
Bone marrow
Bone Marrow Cells - cytology
Bone Marrow Transplantation
Cell culture
Cell Differentiation
Cells, Cultured
Ceramics
Differentiation
Lumbar spinal fusion
Lumbar Vertebrae
Male
Mesenchymal Stem Cell Transplantation - methods
Mesenchymal Stromal Cells - cytology
Mesenchyme
Nervous system
Neurology
Osteoblastogenesis
Osteoblasts
Rabbits
scaffolds
Spinal Fusion - methods
Stem cells
Transplantation, Autologous
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Summary:Abstract Mesenchymal stem cells (MSCs) have been isolated from various tissues and expanded in culture. MSCs add osteogenic potential to ceramic scaffolds when used together. A spinal fusion rabbit model was used to evaluate whether a pellet of cultured, autologous bone marrow MSCs (BMSCs) with osteogenic differentiation could increase the fusion rate when co-grafted with an autologus bone graft compared to autograft alone. Thirty rabbits were randomly assigned to two groups. Group 1 received bone autograft alone and Group 2 received bone autograft plus a pellet of cultured and differentiated BMSCs. Group 2 rabbits had a bone marrow puncture, after which the BMSC were cultured and osteoblastic differentiation was induced. BMSC cultures were obtained from 12 of 15 rabbits. The 27 rabbits underwent a bilateral, L4–L5 intertransverse fusion with an autograft and in Group 2 rabbits a pellet of differentiated BMSCs was added to the autograft. In Group 1, the fusion rate was 53% (8 of 15 rabbits) and in Group 2 the fusion rate was 0% ( p < 0.05). Adding differentiated BMSCs in a pellet without a scaffold not only failed to increase fusion rate, but completely inhibited bony growth.
ISSN:0967-5868
1532-2653
DOI:10.1016/j.jocn.2009.06.024