Bio-ethanol from water hyacinth biomass: An evaluation of enzymatic saccharification strategy

Biomass feedstock having less competition with food crops are desirable for bio-ethanol production and such resources may not be localized geographically. A distributed production strategy is therefore more suitable for feedstock like water hyacinth with a decentralized availability. In this study,...

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Bibliographic Details
Published in:Bioresource technology 2010-02, Vol.101 (3), p.925-930
Main Authors: Aswathy, U.S., Sukumaran, Rajeev K., Devi, G. Lalitha, Rajasree, K.P., Singhania, Reeta Rani, Pandey, Ashok
Format: Article
Language:English
Subjects:
acid treatment
alkali treatment
Aspergillus niger - enzymology
beta-glucosidase
beta-Glucosidase - chemistry
Bio-ethanol
Biofuel production
biofuels
Biological and medical sciences
Biomass
Biotechnology
Blends
Cellulase - chemistry
cellulases
Eichhornia
Eichhornia - metabolism
Eichhornia crassipes
Energy
enzymatic hydrolysis
Enzymes
Ethanol - chemistry
ethanol production
Ethyl alcohol
Feedstock
Fermentation
Fundamental and applied biological sciences. Psychology
hydrolysates
Hydrolysis
Industrial applications and implications. Economical aspects
Industrial Microbiology - methods
lignocellulose
Lignocellulosic biomass
Models, Statistical
Polysaccharides - chemistry
Pretreatment
raw materials
reducing sugars
renewable energy sources
Saccharification
Saccharomyces cerevisiae
Saccharomyces cerevisiae - enzymology
Strategy
Trichoderma - enzymology
Water hyacinth
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Summary:Biomass feedstock having less competition with food crops are desirable for bio-ethanol production and such resources may not be localized geographically. A distributed production strategy is therefore more suitable for feedstock like water hyacinth with a decentralized availability. In this study, we have demonstrated the suitability of this feedstock for production of fermentable sugars using cellulases produced on site. Testing of acid and alkali pretreatment methods indicated that alkali pretreatment was more efficient in making the sample susceptible to enzyme hydrolysis. Cellulase and β-glucosidase loading and the effect of surfactants were studied and optimized to improve saccharification. Redesigning of enzyme blends resulted in an improvement of saccharification from 57% to 71%. A crude trial on fermentation of the enzymatic hydrolysate using the common baker’s yeast Saccharomyces cerevisiae yielded an ethanol concentration of 4.4 g/L.
ISSN:0960-8524
1873-2976
DOI:10.1016/j.biortech.2009.08.019