Dry-reagent gold nanoparticle-based lateral flow biosensor for the simultaneous detection of Vibrio cholerae serogroups O1 and O139

• Published in: Journal of microbiological methods 2011-09, Vol.86 (3), p.277-282
• Main Authors: Yu, Choo Yee, Ang, Geik Yong, Chua, Ang Lim, Tan, Elina Husni, Lee, Su Yin, Falero-Diaz, Gustavo, Otero, Oscar, Rodríguez, Irelio, Reyes, Fátima, Acosta, Armando, Sarmiento, María E., Ghosh, Santanu, Ramamurthy, Thandavarayan, Yean Yean, Chan, Lalitha, Pathabhiraman, Ravichandran, Manickam
• Format: Article
• Language: English
• Subjects: Antibodies, Monoclonal - immunology; Antigen-Antibody Reactions; Bacteriological methods and techniques used in bacteriology; Bacteriology; Biological and medical sciences; Biosensing Techniques - methods; biosensors; Cholera; Cholera - diagnosis; Cholera - immunology; Colloidal gold nanoparticles; Dipstick; food contamination; Fundamental and applied biological sciences. Psychology; gold; Gold - chemistry; Humans; immunoaffinity chromatography; Immunochromatographic assay; lipopolysaccharides; Lipopolysaccharides - immunology; Metal Nanoparticles - chemistry; Microbiology; Monoclonal antibodies; nanogold; Oryza sativa; pandemic; peptones; rice; secretion; Sensitivity and Specificity; Serologic Tests; serotypes; Serotyping; Vibrio cholerae; Vibrio cholerae O1; Vibrio cholerae O1 - immunology; Vibrio cholerae O1 - isolation & purification; Vibrio cholerae O139 - immunology; Vibrio cholerae O139 - isolation & purification
• ISSN: 0167-7012; 1872-8359
• DOI: 10.1016/j.mimet.2011.04.020

Cholera is a communicable disease caused by consumption of contaminated food and water. This potentially fatal intestinal infection is characterised by profuse secretion of rice watery stool that can rapidly lead to severe dehydration and shock, thus requiring treatment to be given immediately. Epidemic and pandemic cholera are exclusively associated with Vibrio cholerae serogroups O1 and O139. In light of the need for rapid diagnosis of cholera and to prevent spread of outbreaks, we have developed and evaluated a direct one-step lateral flow biosensor for the simultaneous detection of both V. cholerae O1 and O139 serogroups using alkaline peptone water culture. Serogroup specific monoclonal antibodies raised against lipopolysaccharides (LPS) were used to functionalize the colloidal gold nanoparticles for dual detection in the biosensor. The assay is based on immunochromatographic principle where antigen–antibody reaction would result in the accumulation of gold nanoparticles and thus, the appearance of a red line on the strip. The dry-reagent dipstick format of the biosensor ensure user-friendly application, rapid result that can be read with the naked eyes and cold-chain free storage that is well-suited to be performed at resource-limited settings.