Manganese-guided cellular MRI of human embryonic stem cell and human bone marrow stromal cell viability

This study investigated the ability of MnCl2 as a cellular MRI contrast agent to determine the in vitro viability of human embryonic stem cells (hESC) and human bone marrow stromal cells (hBMSC). Basic MRI parameters including T1 and T2 values of MnCl2‐labeled hESC and hBMSC were measured and viabil...

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Bibliographic Details
Published in:Magnetic resonance in medicine 2009-10, Vol.62 (4), p.1047-1054
Main Authors: Yamada, Mayumi, Gurney, Paul T., Chung, Jaehoon, Kundu, Pratima, Drukker, Micha, Smith, Alan K., Weissman, Irving L., Nishimura, Dwight, Robbins, Robert C., Yang, Phillip C.
Format: Article
Language:English
Subjects:
Apoptosis
Bioluminescence
Bone marrow
Calcium channels
Cell Line
Cell Survival
cellular MRI
Chlorides
Contrast Media
Embryo cells
Embryonic Stem Cells - cytology
Humans
Image Enhancement - methods
Magnetic resonance imaging
Magnetic Resonance Imaging - methods
Manganese
Manganese Compounds
Mesenchymal Stromal Cells - cytology
N.M.R
Reproducibility of Results
Sensitivity and Specificity
Stem cells
stromal cells
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Summary:This study investigated the ability of MnCl2 as a cellular MRI contrast agent to determine the in vitro viability of human embryonic stem cells (hESC) and human bone marrow stromal cells (hBMSC). Basic MRI parameters including T1 and T2 values of MnCl2‐labeled hESC and hBMSC were measured and viability signal of manganese (Mn2+)‐labeled cells was validated. Furthermore, the biological activity of Ca2+‐channels was modulated utilizing both Ca2+‐channel agonist and antagonist to evaluate concomitant signal changes. Metabolic effects of MnCl2‐labeling were also assessed using assays for cell viability, proliferation, and apoptosis. Finally, in vivo Mn2+‐guided MRI of the transplanted hESC was successfully achieved and validated by bioluminescence imaging. Magn Reson Med, 2009. © 2009 Wiley‐Liss, Inc.
ISSN:0740-3194
1522-2594
1522-2594
DOI:10.1002/mrm.22071